LOCUS       sequence-9259-ex       13687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 13687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC452
FEATURES             Location/Qualifiers
     source          1..13687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    91..111
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(4087..4107)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(4132..4179)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4255..4275
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4297..4317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4339..4359
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4381..4401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4444..4472)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5220..5240
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(6086..6133)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    6201..6225
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7022..7046)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7656..7680
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8477..8501)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(9392..9403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11438..11542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11543..11611,11612..12403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11761..11780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12574..13162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13063..13082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13316..13333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13450..13471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13486..13491,13492..13509,13510..13516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13524..13540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13529..13551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13548..13564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13548..13564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcctcattac gccaagctat caactttgta tagaaaagtt ggcggcgagg
      121 tcggcggtac gggcgaagaa cttgcgagac aggcggatga ttgaggcttc acactgaaaa
      181 tggaaagtta agctaagaat taagcagatg tttcgaaaaa tattcaaaaa tacaatttga
      241 actagacagt agacctaaga ttgaaaagtt ctgattttca ggtggtctcg aaataaacat
      301 tggtacttaa aacatgtgtt tgcaaatgta tagggtgtca ctaaagtttt gtgtctcaga
      361 atgtagtttt gtgacttcac tgccattggt acgagtcatc tcaccatagt tccaagagca
      421 gttggaagct tttgacagac gttcgagtag agtccgtcaa cagcgtgaga gtgaagagcc
      481 tcttggtttt gagcaaagaa gtgctcgaca gctttacagt tttcacatcc caacataacc
      541 ttctctgaca tggccatttc tacgagagct ggaaattgaa aaattacagg atttcagatg
      601 atcgattaac ttaccatttt ccttcttttc gcatgaatgc atcattgtgc agattcctct
      661 tccatcaaat tgattgtaag tcaagaaaag cacagttgac atatacatat gaacaaagtc
      721 attgcatcca tctgtccagt tggcaaccat cttattgcag gcaactgttt ggatgtcatc
      781 agctgttcct tgctgaaaat ataaaccttt taaaactgct tccaacatac ttttacattt
      841 tcttttcaat acatgcagtg gttattgaaa tttgcaacac aattttctac tgcacttact
      901 ctcttatcaa tgaattcctg aagaagagca tcagcggaaa gtgtgcactc gaagcaagac
      961 ataagctcct ctccgttgct tgtcttcacc attcccatag acttccacat ctcagatgct
     1021 ggttgtttcg gagcttcacg agcagctggc ttggatgtag cagagcagag tcccatgttt
     1081 gagcacataa gttccttttc ggtgaacata tccttcatct cggtcataag atctggaatg
     1141 acggaggaga caaagatgtt gcatgccttg tgggcttttc cggcgctctt gcagacaaag
     1201 cgttgaaggg tagatttgac ctgaaacttg agataatttt accgttcttc ctgcaaaatt
     1261 taactcactg cataggtagt aaattcatcc ccaacgagtt ttccgatctg tgcagttgat
     1321 gcctgacatt cgtcacaaac ttcttgtctc tataaaaatt aattgaaaat taaaaattaa
     1381 tctcccaaaa tgacaaacca taatgttatc attagctacg atggcctctg atttcttcaa
     1441 gtaaagcatg gcaagacgag caagtggtga gaattgagat tcaccgcaca tttggagctt
     1501 tgagcataca gcggaggtgt cagcctgaaa attcagagta ctctctgaaa aaatgtcgta
     1561 aacctaccaa gtatggagca agtttgtagg cgatgaagtc aagtttgtca agagtctttg
     1621 agcagtcaga ttgatgagat gtacctacgc agagcattga aagagaaacc ttcagttgag
     1681 ccatcttcat gcgatcctta gaagcatcaa tgaagttttg aaccatctga aatttgttta
     1741 ctttatagta gtaacattta gttctctgaa tataattact taccgatttg catgattcac
     1801 atggagatgg agcagattgt gctccaagag ccaaagctgc cagcacggcg aagacaaaaa
     1861 tggatttcat tactgcaatt tcaaatttat ttgaaataat ttgtaagaaa ttaatgggaa
     1921 actatgaaga aaactgtgcg aaaaatgaag cggaaaactg aaaacacgtg ttactcggtg
     1981 ctccttttat cacctcttat cagtgtgctc ctccgacgtg tgcctctaat ctgatttatc
     2041 aggtaattta tggtgaagcc cactatacaa cactagagta tgacccggtt tttgattgtt
     2101 gttaacacga ggaaacttca aaaaaataca aaaattccac gaaattgttt atcacaggta
     2161 attgaaaaag tcatcattgt gctcactgaa gcttgaaaaa aaaagagcaa aaattagagc
     2221 aatgtggtat gcgacgttgg tgagagaacg ttcaaggaaa tatgcagata ctggttttta
     2281 tggaaatttt cacagcgtta ttttccggtg cttcataaat ttgaaacagt ctgagtgaca
     2341 ctttggcact cattcagtac tctatgagaa ccaacatact tcgttttacc tttctagaaa
     2401 tttaaaacat ttcgataatt tttctgcaac tgccgaatgg tctagtcgac atctcatctc
     2461 atttttcaac tactataact ttttagactt tttataagaa agccagtaat gtcgtaagct
     2521 ttacaatttt caacttgtgg ataaataatt gtgaactttt tcaaaataac aagcgcaata
     2581 acttcaaatc cacataatga cataccacgt ttcctctact tgttcaatag tctcacactg
     2641 atactgagaa gtcttacgtt tcagaaaggt tggtagagtt gccattaaaa acttcaatgt
     2701 gctcgaaatg ctgataaatt ttggaaagtt ctgtgtgttt ttttactttt aaacggtgtc
     2761 gaatgaaggt tttgtaaaac atcgattgtt tttgccacta ataaggtatg ttttcattgt
     2821 ccataagtta ttgatcaact ttcaattttt ctgatagttc agcaatgttg tagctatttg
     2881 tcatgaaaat gatttattct ttaaacagtt tatcagttat cagcaagcag agaaatgaag
     2941 aagtttgatg ttttcaggtc tagcagttca tctggaatat tttaattaaa atcgagcatt
     3001 cgaataccca aactcacagc ctgactgaat ttgttttggc ttgcaaggac tgcggtcaaa
     3061 ctgacaaatc ttggagcaac agatgacatg atttgttgac actgaaaatt gagatttaag
     3121 ctttagaaca ttaaaaaaaa ctaataaaaa cttacacttg gttggaagaa tgataataat
     3181 ccagagcaga aagtctcagt cagtgagttt tcgaagaatt tctccatggc acttccaaca
     3241 aggtttgagc tcaaatatgt ttccaattcc atcattttcg gacatttttc agcttgaatc
     3301 tcttggaatg tgaaatcacg aagtggggca actgaaaaat agtcggtcga ttattggaca
     3361 cctcattatt acagcaaacc tgaacttgct ggacaacact tgttagctag acaaactcct
     3421 tgtggagttg cctgttcaac tgtcatctga ataacagttg ggagatataa atccaagaaa
     3481 tcaatacatg aatcagtcat gttggctgtg acaatcttgt agcatgcgaa atcacggaca
     3541 tcatcggaaa gtttcttctg aaatcactag aggtgaatca tttaaaaatt tgttttggtg
     3601 taggtgattg aaataaacaa atacacagtt ggattgttgt ttttatgtta cgaaactatt
     3661 ttaattgttt ttgcaataaa cttactctgt tagtgatcat gtctgtttgc atgtcttcaa
     3721 caacaaactt gcattcgaaa caggacatgg cgagagtctg aaaaatggga gtgtattgct
     3781 tctattcaaa ttttgaaaaa taagaaacca catcaaattc cctttttctt gcaaaataac
     3841 aacgaaatct ctttccatct ataaacttat gagcaatatc atcatgttcc aattatttac
     3901 cgactttgtc tcgacaatgt cagtagaagg gtaacatgtg tgaagcaggt ggctgttggg
     3961 acggcacacg attccttttt tagatggtaa agggtcagtt acatctgtca ttttcttttc
     4021 attactcaac tcctacttgg ctcacaactc gtcatttttt atagcgtcat cttacacaat
     4081 tttattcaac tttattatac atagttgata attcactggg ctagccgctc tgaagttcct
     4141 atactttcta gagaatagga acttcggaat aggaacttca agatccccgg gtatgtttcg
     4201 aatgatacta acataacata gaacattttc aggagctcag aaaaaatgac tgctccaaag
     4261 aagaagcgta aggtaccggc agaaaaaatg actgctccaa agaagaagcg taaggtaccg
     4321 gcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac cggcagaaaa aatgactgct
     4381 ccaaagaaga agcgtaaggt accggtagaa aaaatgagta aaggagaaga acttttcact
     4441 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     4501 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     4561 actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat
     4621 tatttaaatt ttcagccaac acttgtcact actttctgtt atggtgttca atgcttctcg
     4681 agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
     4741 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa
     4801 cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa
     4861 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     4921 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     4981 aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac taactaatct
     5041 gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     5101 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     5161 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     5221 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggacga actatacaaa
     5281 agcccacaag ctttggaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca
     5341 acgtcgttga attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt
     5401 tattctcttt gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca
     5461 tgagcggttt tttttctctt tccgtctccc aattcgtatt ccgctcctct catctgaaca
     5521 caatgtgcaa gtttatttat cttctcgctt tcatttcatt aggacgtggg gggaattggt
     5581 ggaaggggga aacacacaaa aggatgatgg aaatgaaata aggacacaca atatgcaaca
     5641 acattcaatt cagaaatatg gaggaaggtt taaaagaaaa cataaaaata tatagaggag
     5701 gaaggaaaac tagtaaaaaa taagcaaaga aattaggcga acgatgagaa ttgtcctcgc
     5761 ttgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5821 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     5881 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     5941 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     6001 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6061 ataataatgg tttcttagac gctctgaagt tcctatactt tctagagaat aggaacttcg
     6121 gaataggaac ttcaagatcc ccgggtatgt ttcgaatgat actaacataa catagaacat
     6181 tttcaggagg atccctcgag acaagtttgt acaaaaaagc aggcatggcg aagaaaccga
     6241 gagttaccaa gctgaatacg gatcgaagat cgagagcaaa cgagtacgtt ctcaaattaa
     6301 ttacgataaa attaacattt tcttgaattt ttttagtagt taatacgact cctagcaata
     6361 aaattgaata taatatgaaa attagttaga aaaaaatata ccggtcagaa ctcaagcttc
     6421 atgaagaact gtttctagat agaatgcccc acatttttca atatctcaga aatcacatgc
     6481 ttcatcatca aaggtttatt aaacattgta aactttcaga cgagaacgac agagagtttc
     6541 cgagatgaac ggaatgtttg atgttctact taatcttctt ccgccgtcac attttaaaac
     6601 acgactctcc agagttcaaa tcttgaggta agttccccaa taagatgaga tctcttttag
     6661 aaaattttca aaactctacg gaagtttact tgtttgtttt cttaattcaa agattttcat
     6721 taaaaaaata accctcgcat ttcagagaag ccacctctta cataattcgt cttcacaact
     6781 ttcttattga aagttcaaat agtgacatag acgcaataac tgtatttccg cacatcttca
     6841 acggagaacg caagtcaaat aaagatgcaa ttcgtcgtcc catgaagttg aagcagggtg
     6901 gcggtgttgc tgctttcatc tcacgccacg aacttccccc acttcaactt ccaaatcccg
     6961 ttatcccaat tttgaagccc acttcagttc cagtttggcc ccaaaccaat gtttatattg
     7021 cacccagctt tcttgtacaa agtggtctcg agaggcctgt tagccaactc tagagtactc
     7081 ctacagtacc cctatagtac agctacagta cctcgcacta tccctccatc aattaatttt
     7141 acagtaatcc tacagtaccc catacagtat cactacaaca cctcctacta tccctccacc
     7201 aactacttcc acagttgtcc tacagtaccc ctacagtatc actgcagtac ctactactat
     7261 ctcatcacca actactccta cagtaatcct aaagtaccat gcactattcc tccatcaact
     7321 aaccttacag taatcctaca gtaccgctac agtacctcat actatccctc caccaattaa
     7381 tcttacagtt gtcctacagt accccataca gtatcactac agtacctcct actatatcac
     7441 caccaactac ttctacagta ctcctacagt acctctatag tgcagctaca gtacctcata
     7501 ctatcccacc aacaactaat attacagtaa tcctacagta ccccatacag tacctctaca
     7561 ctgcccccgc catgcaatat gaacatatca atagtagaaa aaaatccaat tacagaggcc
     7621 ttgatcagcg gccgcgggaa ttcgattggg tctagaccac tttgtacaag aaagctgggt
     7681 gcaatataaa cattggtttg gggccaaact ggaactgaag tgggcttcaa aattgggata
     7741 acgggatttg gaagttgaag tgggggaagt tcgtggcgtg agatgaaagc agcaacaccg
     7801 ccaccctgct tcaacttcat gggacgacga attgcatctt tatttgactt gcgttctccg
     7861 ttgaagatgt gcggaaatac agttattgcg tctatgtcac tatttgaact ttcaataaga
     7921 aagttgtgaa gacgaattat gtaagaggtg gcttctctga aatgcgaggg ttattttttt
     7981 aatgaaaatc tttgaattaa gaaaacaaac aagtaaactt ccgtagagtt ttgaaaattt
     8041 tctaaaagag atctcatctt attggggaac ttacctcaag atttgaactc tggagagtcg
     8101 tgttttaaaa tgtgacggcg gaagaagatt aagtagaaca tcaaacattc cgttcatctc
     8161 ggaaactctc tgtcgttctc gtctgaaagt ttacaatgtt taataaacct ttgatgatga
     8221 agcatgtgat ttctgagata ttgaaaaatg tggggcattc tatctagaaa cagttcttca
     8281 tgaagcttga gttctgaccg gtatattttt ttctaactaa ttttcatatt atattcaatt
     8341 ttattgctag gagtcgtatt aactactaaa aaaattcaag aaaatgttaa ttttatcgta
     8401 attaatttga gaacgtactc gtttgctctc gatcttcgat ccgtattcag cttggtaact
     8461 ctcggtttct tcgccatgcc tgcttttttg tacaaacttg tctagaccca atcactagcc
     8521 ggccatacaa gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa attttaaata
     8581 ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt tttcttgctt
     8641 tctaaaaaat taattcaatc caaatctaaa catgagcggt tttttttctc tttccgtctc
     8701 ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt atcttctcgc
     8761 tttcatttca ttaggacgtg gggggaattg gtggaagggg gaaacacaca aaaggatgat
     8821 ggaaatgaaa taaggacaca caatatgcaa caacattcaa ttcagaaata tggaggaagg
     8881 tttaaaagaa aacataaaaa tatatagagg aggaaggaaa actagtaaaa aataagcaaa
     8941 gaaattaggc gaacgatgag aattgtcctc gcttgggccc tttcgtctcg cgcgtttcgg
     9001 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     9061 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     9121 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     9181 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg
     9241 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acaacggata
     9301 aaatgtttat tataatatca aaaacaattt gtcagttgac cactttttga tatatttgac
     9361 agaaaccggg atgaattggc tcaaagttag ggcgcccttc tattacaggg tttctgataa
     9421 caaaccggtt attaactccc aacaagggat gatttcaatt catcatgctc aaattgaccc
     9481 aaattaagtt acatgacaaa ttcatcgccc ttttcaactc ttcttggctc atcatctgtt
     9541 attctgttcc tattctgtct gcaccctata cccctttgca tactctctcg tcattccttt
     9601 cttggatagt gtcttcttct ccccagctct gctacttcta tgacttgcgc ccgctgcttt
     9661 tccgctcgtt cctctcctct ctgacgtctt cttctgcttc ttttctcctt cctttttccc
     9721 ttctcaatct tcctcttttt cccatttctg tcaatcattc gaagaagaag aagaagaaga
     9781 ccctcattca tttcattttt ttttctgtcg gtgtgtgctg ctcggttaag agtgagctct
     9841 ctattccacg tcttcttctt tttcttcttc gattcgaatc aatcactcca ccaaaacgca
     9901 ttcgtttttg ggattcaccc cgcggttcgc aacaggtttc tttttcaaat tattagcgtt
     9961 ataaatagaa aaatggtgga gtttcaataa aaatgataat ttacaaagtg tattttgatt
    10021 acatgtactc aaaaaggttg aaatttttca taccagtttt ccggaaatcc atctgatatc
    10081 attatcgtat ttttcttttt aaaaatgttt tcaaaaaaaa acaaaatata gctggttatt
    10141 tggcaccctc taattaccat tttcctgtca caccacactc ttttccttct tccacttctt
    10201 tccgttttca gccgcttcca accaaaccga tatgaaagcc gagcaacaac aatcgattcc
    10261 acccggctcg gcgaccttcc cgtcgcaggt gagactcaga aaactagaga aaccgctcaa
    10321 ctaactcttg atatccgatt tcattctttt ctcttttctt tttgttgaac ttttccatat
    10381 ttccagatgc cacggccacc accaagcacc gaacaaggaa tcacaacgga atcggagctt
    10441 gcgaagaaag ctcaaatcac tccgaacgac gttttagcac ttccgggaat cactcaaggt
    10501 atcttgctcc ctttggattt cagtttaaaa cataaattta ggattcttat gctccccatc
    10561 tgcgaacatc tataacatcg agttcaccaa gttccaaatc cgtgatctgg acactgagca
    10621 agtgcttttc gagatcgcca aaccggagaa cgatcaggag aatgatgagt cgccacagga
    10681 gtcggcaaga tacgtgcgtt atagatttgc tccaaacttt ttgaaactca aaacggtcgg
    10741 agcaactgtg gaattcaaag taggagacat cccaatccat catttccgaa tgatcgaacg
    10801 tcacttcttc aaagatcgcc ttctgaagtg ttttgacttt gaattcggat tctgtattcc
    10861 gaattcacga aacaactgtg aacatatcta tgagttccct caactctctc aacaactcag
    10921 tgagtcatta ttctaaaaag tacaattcaa aagactaatc tctttttcag tggacgacat
    10981 gatcaacaat ccaaacgaga ctcgttctga cagcttctat ttcgtcgata acaaactcgt
    11041 catgcacaac aaagccgact actcatatga tgcataaata tttaatacaa aaatgttctg
    11101 gataattatt ctgtcgaata gaaaaaaaac tccaaatgtg attaaattcc aataattcct
    11161 gtctagtttg ttccttcccc ttccccttct catgttcaat gcattcctaa gcttttcagt
    11221 tccccccttg tttctatatt ttttcgctgt cctgtcacac tcgctaaaaa cactaatcac
    11281 acggaaatct gttttcaata aaaactccaa ctttaactca ttttcaattt caactgaaag
    11341 attttttcat tagagaatgt ctaggcatgc tctagagggc ccaaaaggcc tttttttttt
    11401 ggcgccgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    11461 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    11521 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    11581 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    11641 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    11701 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    11761 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
    11821 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    11881 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    11941 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    12001 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    12061 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
    12121 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    12181 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    12241 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    12301 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    12361 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    12421 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    12481 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    12541 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    12601 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    12661 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    12721 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    12781 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    12841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    12901 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    12961 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    13021 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    13081 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    13141 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    13201 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    13261 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    13321 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    13381 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    13441 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    13501 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    13561 tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa ctattctcat
    13621 ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata cgctaacaac
    13681 ttggaaa
//
