LOCUS       sequence-9260-ex       13226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 13226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC93
FEATURES             Location/Qualifiers
     source          1..13226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     443..459
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     444..460
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    475..599
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(624..629,630..647,648..654)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             708..1367
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(774..793)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1709..2014
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1893..1912
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2055..2179)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2218..2265)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2304..2324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2346..2366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2388..2408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2430..2450
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2493..2521)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3269..3289
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(3931..3942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5932..5979)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6096..6116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6206..6228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10666..10688)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10788..10807
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10885..10903)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10971..11075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11076..11144,11145..11936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11294..11313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12107..12695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12596..12615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12849..12866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12983..13004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13019..13024,13025..13042,13043..13049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13057..13073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13062..13084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13081..13097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13081..13097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagtttgt gcaacaaatt gaggaaggca ttctagagaa gaatttcggc
       61 atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa ccggtttgaa
      121 ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac aataaattca
      181 caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata catctagaat
      241 cctttgctgt agaagaattg ctcaataaga acacgtggag ctcctttaat tttgatacga
      301 cctttcaact catcgacgtg actggcgatc gaatgtccaa gatgttcttc aagataggag
      361 cggagatcat tttcgcaagc ctgaaagata tttatgttga ttcaggattt aataatgttc
      421 ccaacttaca aaaatgtctc cgtcgaggtc gacggtatcg ataagcttga tatcacaagt
      481 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
      541 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcatattgg
      601 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
      661 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      721 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      781 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      841 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      901 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
      961 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1021 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1081 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1141 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1201 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1261 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1321 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg
     1381 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     1441 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     1501 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     1561 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     1621 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     1681 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     1741 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     1801 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     1861 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     1921 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     1981 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     2041 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagcattat gtagtctgtt
     2101 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     2161 ctttcttgta caaagtggtg atatcagatc cactagttct agcttcaaaa gcgctctgaa
     2221 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat ccccgggtac
     2281 cgagctcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggca gaaaaaatga
     2341 ctgctccaaa gaagaagcgt aaggtaccgg cagaaaaaat gactgctcca aagaagaagc
     2401 gtaaggtacc ggcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggtagaaa
     2461 aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     2521 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     2581 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tgggtaagtt
     2641 taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca cttgtcacta
     2701 ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa cggcatgact
     2761 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     2821 acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca tacatattta
     2881 aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     2941 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     3001 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt
     3061 taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc aaaattagac
     3121 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     3181 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     3241 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     3301 ttacacatgg catggacgaa ctatacaaaa gcccacaagc tttggaattc gctagccggc
     3361 catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg
     3421 aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct
     3481 aaaaaattaa ttcaatccaa atctaaacat gagcggtttt ttttctcttt ccgtctccca
     3541 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     3601 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     3661 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     3721 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtctagaat cctatgcttg
     3781 cactttgagc caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa
     3841 aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca
     3901 gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac
     3961 aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca
     4021 aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta
     4081 ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc
     4141 ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt
     4201 ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct
     4261 tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac
     4321 cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc
     4381 tattccacgt cttcttcttt ttcttcttcg attcgaatca atcactccac caaaacgcat
     4441 tcgtttttgg gattcacccc gcggttcgca acaggtttct ttttcaaatt attagcgtta
     4501 taaatagaaa aatggtggag tttcaataaa aatgataatt tacaaagtgt attttgatta
     4561 catgtactca aaaaggttga aatttttcat accagttttc cggaaatcca tctgatatca
     4621 ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa caaaatatag ctggttattt
     4681 ggcaccctct aattaccatt ttcctgtcac accacactct tttccttctt ccacttcttt
     4741 ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg agcaacaaca atcgattcca
     4801 cccggctcgg cgaccttccc gtcgcaggtg agactcagaa aactagagaa accgctcaac
     4861 taactcttga tatccgattt cattcttttc tcttttcttt ttgttgaact tttccatatt
     4921 tccagatgcc acggccacca ccaagcaccg aacaaggaat cacaacggaa tcggagcttg
     4981 cgaagaaagc tcaaatcact ccgaacgacg ttttagcact tccgggaatc actcaaggta
     5041 tcttgctccc tttggatttc agtttaaaac ataaatttag gattcttatg ctccccatct
     5101 gcgaacatct ataacatcga gttcaccaag ttccaaatcc gtgatctgga cactgagcaa
     5161 gtgcttttcg agatcgccaa accggagaac gatcaggaga atgatgagtc gccacaggag
     5221 tcggcaagat acgtgcgtta tagatttgct ccaaactttt tgaaactcaa aacggtcgga
     5281 gcaactgtgg aattcaaagt aggagacatc ccaatccatc atttccgaat gatcgaacgt
     5341 cacttcttca aagatcgcct tctgaagtgt tttgactttg aattcggatt ctgtattccg
     5401 aattcacgaa acaactgtga acatatctat gagttccctc aactctctca acaactcagt
     5461 gagtcattat tctaaaaagt acaattcaaa agactaatct ctttttcagt ggacgacatg
     5521 atcaacaatc caaacgagac tcgttctgac agcttctatt tcgtcgataa caaactcgtc
     5581 atgcacaaca aagccgacta ctcatatgat gcataaatat ttaatacaaa aatgttctgg
     5641 ataattattc tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca ataattcctg
     5701 tctagtttgt tccttcccct tccccttctc atgttcaatg cattcctaag cttttcagtt
     5761 ccccccttgt ttctatattt tttcgctgtc ctgtcacact cgctaaaaac actaatcaca
     5821 cggaaatctg ttttcaataa aaactccaac tttaactcat tttcaatttc aactgaaaga
     5881 ttttttcatt agagaatgtc taggcatgca agctagcttc aaaagcgctc tgaagttcct
     5941 atactttcta gagaatagga acttcggaat aggaacttca agatccccgg gattggccaa
     6001 aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc
     6061 cttggagggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
     6121 tgggtgaaga ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg
     6181 cgctgtatgg cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6241 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6301 aggcccgcac cgatcgccct tcccaacagt tgcgaaggta agtttaaaca gatccatact
     6361 aactaacttg ttctgacata attttcagct tgaatggcga atggcgcttt gcctggtttc
     6421 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
     6481 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa
     6541 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
     6601 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
     6661 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
     6721 taagtttaat taagttgata ctaactaaca aagatctgat taattttcag gacagtcgtt
     6781 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
     6841 tggtgctgcg ttggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
     6901 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
     6961 ttgccactcg ctttaatgat gatttctccc gcgctgtact ggaggctgaa gtccaggtaa
     7021 gtttaaacag gatcttacta actaacatgc taacactgaa ttttcagatg tgcggcgagt
     7081 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg
     7141 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca
     7201 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc
     7261 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg
     7321 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
     7381 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
     7441 agacgatggt gcaggatgta agtttaaact attcgttact aactaacttt aaacatttaa
     7501 attttcagat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
     7561 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
     7621 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
     7681 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
     7741 tgatcatctg gtcgctgggg aatggtaagt ttaaacagtt gaatactaac taacggagat
     7801 ctttgaaatt ttcagaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca
     7861 aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca
     7921 ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc
     7981 cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgattcttt
     8041 gcgaggtaag tttaaacaga actctactaa ctaacacatt agatcctaat tttcagtacg
     8101 ctcacgcgat gggcaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt
     8161 atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg
     8221 atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc
     8281 gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgtaagttt
     8341 aaacaataac ctactaacta acgtagataa tttaaatttt caggctgacg gaagcaaaac
     8401 accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat
     8461 acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc
     8521 cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac
     8581 tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc
     8641 aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcatggcag cagtggaggt
     8701 aagtttaaac aagatcctac taactaactc tacattgatg aattttcaga ctggcggaaa
     8761 acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa
     8821 tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc
     8881 tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca
     8941 cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg
     9001 cctgggtcgg taagtttaaa caaagttgta ctaactaacg aagatcttga taattttcag
     9061 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     9121 atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     9181 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     9241 ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     9301 tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     9361 accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtagtaag
     9421 tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagtacc ccgtacgtct
     9481 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
     9541 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca
     9601 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
     9661 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc caactgagcg
     9721 ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag
     9781 agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc
     9841 gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc
     9901 ctgtgctccc accccctatt tttgttatta tcaaaaaaac ttcttcttaa tttctttgtt
     9961 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
    10021 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
    10081 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttttacttc tgataaattt
    10141 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
    10201 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
    10261 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
    10321 attaattttc ctgcttttgc tttttggggg tttcccctat tgtttgtcaa gagtttcgag
    10381 gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga
    10441 agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga
    10501 gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata
    10561 actgtttcct actagtaaaa aataagcaaa gaaattaggc gaacgatgag aattgtcctc
    10621 gcttgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10681 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10741 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10801 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10861 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
    10921 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10981 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    11041 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    11101 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    11161 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    11221 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    11281 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    11341 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    11401 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    11461 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    11521 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    11581 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    11641 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    11701 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    11761 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    11821 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    11881 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    11941 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    12001 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    12061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    12121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    12181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    12241 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    12301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    12361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    12421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    12481 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    12541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    12601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    12661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    12721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    12781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    12841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    12901 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    12961 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    13021 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    13081 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
    13141 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
    13201 atacgctaac aacttggaaa tgaaat
//
