LOCUS       sequence-92605-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from HSC1-GiP where 2 copies of the HS4 insulator core 
            inside the 3'LTR and the D4Z4-c insulator, are combined upstream of 
            the EF1 alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Rival-Gervier S, Lo MY, Khattak S, Pasceri P, Lorincz MC, Ellis J
  TITLE     Kinetics and epigenetics of retroviral silencing in mouse embryonic 
            stem cells defined by deletion of the D4Z4 element.
  JOURNAL   Mol Ther. 2013 Aug;21(8):1536-50. doi: 10.1038/mt.2013.131. Epub 
            2013 Jun 11.
  PUBMED    23752310
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2013.131"; journalName: "Mol Ther"; date: "2013-08"; 
            volume: "21"; issue: "8"; pages: "1536-50"
COMMENT     Sequence Label: HSC1-HS4-D4Z4c-GiP
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    217..250
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
       61 ccgaccgaaa ggagcgcacg accccatgca tcgatcatat ggataaaata aaagatttta
      121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagga
      181 gctctcccat atggtcgacg gtatcgccgg ggatctataa cttcgtataa tgtatgctat
      241 acgaagttat agatcctcta gcgataagct tgatatcgaa ttcgagttgg cgcgcctgtc
      301 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg
      361 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
      421 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
      481 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
      541 tttgggaggg ggctgtcccc gtgagctccc aggcgcgcct gtcattctaa atctctcttt
      601 cagcctaaag ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
      661 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
      721 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
      781 ctgcccccta
//
