LOCUS       sequence-9261-ex       10514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10514)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC97
FEATURES             Location/Qualifiers
     source          1..10514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     443..459
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     444..460
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    475..599
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(624..629,630..647,648..654)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             708..1367
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(774..793)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1709..2014
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1893..1912
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2055..2179)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2218..2265)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2304..2324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2346..2366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2388..2408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2430..2450
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2493..2521)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3269..3289
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(3931..3942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5975..6022)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6051..6728
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             6771..7445
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(7954..7976)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8076..8095
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8173..8191)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8259..8363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8364..8432,8433..9224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8582..8601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9395..9983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9884..9903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10137..10154
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10271..10292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10307..10312,10313..10330,10331..10337)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10345..10361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10350..10372
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10369..10385
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10369..10385
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagtttgt gcaacaaatt gaggaaggca ttctagagaa gaatttcggc
       61 atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa ccggtttgaa
      121 ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac aataaattca
      181 caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata catctagaat
      241 cctttgctgt agaagaattg ctcaataaga acacgtggag ctcctttaat tttgatacga
      301 cctttcaact catcgacgtg actggcgatc gaatgtccaa gatgttcttc aagataggag
      361 cggagatcat tttcgcaagc ctgaaagata tttatgttga ttcaggattt aataatgttc
      421 ccaacttaca aaaatgtctc cgtcgaggtc gacggtatcg ataagcttga tatcacaagt
      481 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
      541 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcatattgg
      601 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
      661 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      721 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      781 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      841 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      901 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
      961 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1021 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1081 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1141 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1201 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1261 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1321 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg
     1381 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     1441 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     1501 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     1561 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     1621 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     1681 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     1741 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     1801 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     1861 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     1921 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     1981 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     2041 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagcattat gtagtctgtt
     2101 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     2161 ctttcttgta caaagtggtg atatcagatc cactagttct agcttcaaaa gcgctctgaa
     2221 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat ccccgggtac
     2281 cgagctcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggca gaaaaaatga
     2341 ctgctccaaa gaagaagcgt aaggtaccgg cagaaaaaat gactgctcca aagaagaagc
     2401 gtaaggtacc ggcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggtagaaa
     2461 aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     2521 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     2581 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tgggtaagtt
     2641 taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca cttgtcacta
     2701 ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa cggcatgact
     2761 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     2821 acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca tacatattta
     2881 aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     2941 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     3001 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt
     3061 taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc aaaattagac
     3121 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     3181 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     3241 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     3301 ttacacatgg catggacgaa ctatacaaaa gcccacaagc tttggaattc gctagccggc
     3361 catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg
     3421 aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct
     3481 aaaaaattaa ttcaatccaa atctaaacat gagcggtttt ttttctcttt ccgtctccca
     3541 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     3601 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     3661 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     3721 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtctagaat cctatgcttg
     3781 cactttgagc caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa
     3841 aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca
     3901 gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac
     3961 aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca
     4021 aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta
     4081 ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc
     4141 ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt
     4201 ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct
     4261 tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac
     4321 cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc
     4381 tattccacgt cttcttcttt ttcttcttcg attcgaatca atcactccac caaaacgcat
     4441 tcgtttttgg gattcacccc gcggttcgca acaggtttct ttttcaaatt attagcgtta
     4501 taaatagaaa aatggtggag tttcaataaa aatgataatt tacaaagtgt attttgatta
     4561 catgtactca aaaaggttga aatttttcat accagttttc cggaaatcca tctgatatca
     4621 ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa caaaatatag ctggttattt
     4681 ggcaccctct aattaccatt ttcctgtcac accacactct tttccttctt ccacttcttt
     4741 ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg agcaacaaca atcgattcca
     4801 cccggctcgg cgaccttccc gtcgcaggtg agactcagaa aactagagaa accgctcaac
     4861 taactcttga tatccgattt cattcttttc tcttttcttt ttgttgaact tttccatatt
     4921 tccagatgcc acggccacca ccaagcaccg aacaaggaat cacaacggaa tcggagcttg
     4981 cgaagaaagc tcaaatcact ccgaacgacg ttttagcact tccgggaatc actcaaggta
     5041 tcttgctccc tttggatttc agtttaaaac ataaatttag gattcttatg ctccccatct
     5101 gcgaacatct ataacatcga gttcaccaag ttccaaatcc gtgatctgga cactgagcaa
     5161 gtgcttttcg agatcgccaa accggagaac gatcaggaga atgatgagtc gccacaggag
     5221 tcggcaagat acgtgcgtta tagatttgct ccaaactttt tgaaactcaa aacggtcgga
     5281 gcaactgtgg aattcaaagt aggagacatc ccaatccatc atttccgaat gatcgaacgt
     5341 cacttcttca aagatcgcct tctgaagtgt tttgactttg aattcggatt ctgtattccg
     5401 aattcacgaa acaactgtga acatatctat gagttccctc aactctctca acaactcagt
     5461 gagtcattat tctaaaaagt acaattcaaa agactaatct ctttttcagt ggacgacatg
     5521 atcaacaatc caaacgagac tcgttctgac agcttctatt tcgtcgataa caaactcgtc
     5581 atgcacaaca aagccgacta ctcatatgat gcataaatat ttaatacaaa aatgttctgg
     5641 ataattattc tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca ataattcctg
     5701 tctagtttgt tccttcccct tccccttctc atgttcaatg cattcctaag cttttcagtt
     5761 ccccccttgt ttctatattt tttcgctgtc ctgtcacact cgctaaaaac actaatcaca
     5821 cggaaatctg ttttcaataa aaactccaac tttaactcat tttcaatttc aactgaaaga
     5881 ttttttcatt agagaatgtc taggcatgcc tgcaggtcga ctctagaccg cggttttggc
     5941 gcggatccac tagttctagc ttcaaaagcg ctctgaagtt cctatacttt ctagagaata
     6001 ggaacttcgg aataggaact tcaagatccc cggtagacga taaggatccg atggtggcct
     6061 cctccgagga cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
     6121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     6181 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     6241 cccagttcca gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgactaca
     6301 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     6361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     6421 agttccgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     6481 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     6541 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
     6601 ccaagaagcc cgtgcagctg cccggctact actacgtgga caccaagctg gacatcacct
     6661 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     6721 tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg
     6781 aggacgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
     6841 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     6901 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     6961 tccagtacgg ctccaaggcg tacgtgaagc accccgccga catccccgac tacaagaagc
     7021 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     7081 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagttcc
     7141 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     7201 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
     7261 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
     7321 agcccgtgca gctgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
     7381 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
     7441 tgtaggaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca acgtcgttga
     7501 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     7561 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tgagcggttt
     7621 tttttctctt tccgtctccc aattcgtatt ccgctcctct catctgaaca caatgtgcaa
     7681 gtttatttat cttctcgctt tcatttcatt aggacgtggg gggaattggt ggaaggggga
     7741 aacacacaaa aggatgatgg aaatgaaata aggacacaca atatgcaaca acattcaatt
     7801 cagaaatatg gaggaaggtt taaaagaaaa cataaaaata tatagaggag gaaggaaaac
     7861 tagtaaaaaa taagcaaaga aattaggcga acgatgagaa ttgtcctcgc ttgggccctt
     7921 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     7981 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     8041 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     8101 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     8161 gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8221 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9541 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9781 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10141 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10201 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10261 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10321 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10381 atgaccatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca
    10441 tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa
    10501 cttggaaatg aaat
//
