LOCUS       sequence-9263-ex       10661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC238
FEATURES             Location/Qualifiers
     source          1..10661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    77..201
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(226..231,232..249,250..256)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             310..969
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(376..395)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1311..1616
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1495..1514
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1657..1781)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1791..1838)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1914..1934
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1956..1976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1998..2018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2040..2060
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2103..2131)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2879..2899
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(3796..3843)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3919..3939
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3961..3981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4003..4023
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4045..4065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4087..4764
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             4807..5481
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     CDS             complement(6366..6377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8412..8516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8517..8585,8586..9377)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8735..8754)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9548..10136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10037..10056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10290..10307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10424..10445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10460..10465,10466..10483,10484..10490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10498..10514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10503..10525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10522..10538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10522..10538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
      121 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      181 acaacatatc cagtcatatt ggcggccgca ttaggcaccc caggctttac actttatgct
      241 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
      301 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      361 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      421 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
      481 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
      541 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
      601 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
      661 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
      721 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
      781 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
      841 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
      901 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
      961 ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     1021 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     1081 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     1141 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     1201 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     1261 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     1321 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     1381 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     1441 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     1501 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     1561 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     1621 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     1681 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     1741 tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatgctct gaagttccta
     1801 tactttctag agaataggaa cttcggaata ggaacttcaa gatccccggg tatgtttcga
     1861 atgatactaa cataacatag aacattttca ggagctcaga aaaaatgact gctccaaaga
     1921 agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
     1981 cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggcagaaaaa atgactgctc
     2041 caaagaagaa gcgtaaggta ccggtagaaa aaatgagtaa aggagaagaa cttttcactg
     2101 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     2161 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     2221 ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact aaccctgatt
     2281 atttaaattt tcagccaaca cttgtcacta ctttctgtta tggtgttcaa tgcttctcga
     2341 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
     2401 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt aagtttaaac
     2461 agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc aagtttgaag
     2521 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     2581 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     2641 aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat tttactaact aactaatctg
     2701 atttaaattt tcagaacttc aaaattagac acaacattga agatggaagc gttcaactag
     2761 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     2821 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
     2881 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggacgaa ctatacaaaa
     2941 gcccacaagc tttggaattc gctagccggc catacaagta atccggatga tcgacgccaa
     3001 cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt
     3061 attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat
     3121 gagcggtttt ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac
     3181 aatgtgcaag tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg
     3241 gaagggggaa acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa
     3301 cattcaattc agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg
     3361 aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat tgtcctcgct
     3421 tgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     3481 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     3541 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     3601 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     3661 ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3721 taataatggt ttcttagacg actctagacc gcggttttgg cgcggatcca ctagttctag
     3781 cttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
     3841 ttcaagatcc ccgggtatgt ttcgaatgat actaacataa catagaacat tttcaggagc
     3901 tcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac cggcagaaaa aatgactgct
     3961 ccaaagaaga agcgtaaggt accggcagaa aaaatgactg ctccaaagaa gaagcgtaag
     4021 gtaccggcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt agacgataag
     4081 gatccgatgg tggcctcctc cgaggacgtc atcaaagagt tcatgcgctt caaggtgcgc
     4141 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4201 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     4261 tgggacatcc tgtcccccca gttccagtac ggctccaagg cgtacgtgaa gcaccccgcc
     4321 gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4381 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcacg
     4441 ctgatctaca aggtgaagtt ccgcggcacc aacttccccc ccgacggccc cgtaatgcag
     4501 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     4561 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     4621 aagaccatct acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggacacc
     4681 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     4741 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc
     4801 ggcaccgcct cctccgagga cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     4861 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4921 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     4981 atcctgtccc cccagttcca gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     5041 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     5101 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     5161 tacaaggtga agttccgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     5221 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     5281 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     5341 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga caccaagctg
     5401 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     5461 cgccaccacc tgttcctgta ggaattcgct agccggccat acaagtaatc cggatgatcg
     5521 acgccaacgt cgttgaattt tcaaatttta aatactgaat atttgttttt tttcctatta
     5581 tttatttatt ctctttgtgt tttttttctt gctttctaaa aaattaattc aatccaaatc
     5641 taaacatgag cggttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc
     5701 tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga
     5761 attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat
     5821 gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata
     5881 gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgagaattgt
     5941 cctcgcttgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     6001 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     6061 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     6121 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6181 gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat
     6241 gtcatgataa taatggtttc ttagacaacg gataaaatgt ttattataat atcaaaaaca
     6301 atttgtcagt tgaccacttt ttgatatatt tgacagaaac cgggatgaat tggctcaaag
     6361 ttagggcgcc cttctattac agggtttctg ataacaaacc ggttattaac tcccaacaag
     6421 ggatgatttc aattcatcat gctcaaattg acccaaatta agttacatga caaattcatc
     6481 gcccttttca actcttcttg gctcatcatc tgttattctg ttcctattct gtctgcaccc
     6541 tatacccctt tgcatactct ctcgtcattc ctttcttgga tagtgtcttc ttctccccag
     6601 ctctgctact tctatgactt gcgcccgctg cttttccgct cgttcctctc ctctctgacg
     6661 tcttcttctg cttcttttct ccttcctttt tcccttctca atcttcctct ttttcccatt
     6721 tctgtcaatc attcgaagaa gaagaagaag aagaccctca ttcatttcat ttttttttct
     6781 gtcggtgtgt gctgctcggt taagagtgag ctctctattc cacgtcttct tctttttctt
     6841 cttcgattcg aatcaatcac tccaccaaaa cgcattcgtt tttgggattc accccgcggt
     6901 tcgcaacagg tttctttttc aaattattag cgttataaat agaaaaatgg tggagtttca
     6961 ataaaaatga taatttacaa agtgtatttt gattacatgt actcaaaaag gttgaaattt
     7021 ttcataccag ttttccggaa atccatctga tatcattatc gtatttttct ttttaaaaat
     7081 gttttcaaaa aaaaacaaaa tatagctggt tatttggcac cctctaatta ccattttcct
     7141 gtcacaccac actcttttcc ttcttccact tctttccgtt ttcagccgct tccaaccaaa
     7201 ccgatatgaa agccgagcaa caacaatcga ttccacccgg ctcggcgacc ttcccgtcgc
     7261 aggtgagact cagaaaacta gagaaaccgc tcaactaact cttgatatcc gatttcattc
     7321 ttttctcttt tctttttgtt gaacttttcc atatttccag atgccacggc caccaccaag
     7381 caccgaacaa ggaatcacaa cggaatcgga gcttgcgaag aaagctcaaa tcactccgaa
     7441 cgacgtttta gcacttccgg gaatcactca aggtatcttg ctccctttgg atttcagttt
     7501 aaaacataaa tttaggattc ttatgctccc catctgcgaa catctataac atcgagttca
     7561 ccaagttcca aatccgtgat ctggacactg agcaagtgct tttcgagatc gccaaaccgg
     7621 agaacgatca ggagaatgat gagtcgccac aggagtcggc aagatacgtg cgttatagat
     7681 ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac tgtggaattc aaagtaggag
     7741 acatcccaat ccatcatttc cgaatgatcg aacgtcactt cttcaaagat cgccttctga
     7801 agtgttttga ctttgaattc ggattctgta ttccgaattc acgaaacaac tgtgaacata
     7861 tctatgagtt ccctcaactc tctcaacaac tcagtgagtc attattctaa aaagtacaat
     7921 tcaaaagact aatctctttt tcagtggacg acatgatcaa caatccaaac gagactcgtt
     7981 ctgacagctt ctatttcgtc gataacaaac tcgtcatgca caacaaagcc gactactcat
     8041 atgatgcata aatatttaat acaaaaatgt tctggataat tattctgtcg aatagaaaaa
     8101 aaactccaaa tgtgattaaa ttccaataat tcctgtctag tttgttcctt ccccttcccc
     8161 ttctcatgtt caatgcattc ctaagctttt cagttccccc cttgtttcta tattttttcg
     8221 ctgtcctgtc acactcgcta aaaacactaa tcacacggaa atctgttttc aataaaaact
     8281 ccaactttaa ctcattttca atttcaactg aaagattttt tcattagaga atgtctaggc
     8341 atgctctaga gggcccaaaa ggcctttttt ttttggcgcc gacgtcaggt ggcacttttc
     8401 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8461 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8521 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8581 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8641 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8701 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8761 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8821 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8881 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8941 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9001 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9061 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9121 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9181 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9241 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9301 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9361 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9421 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9481 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9541 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9601 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9661 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9721 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9781 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9841 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9901 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     9961 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10021 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10081 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10141 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10201 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10261 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10321 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    10381 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    10441 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    10501 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctgtaag
    10561 tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc
    10621 tgaaatcact cacaacgatg gatacgctaa caacttggaa a
//
