LOCUS       sequence-92634-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter plasmid containing 2 myc cDNA binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Gupta S, Seth A, Davis RJ
  TITLE     Transactivation of gene expression by Myc is inhibited by mutation 
            at the phosphorylation sites Thr-58 and Ser-62.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3216-20.
  PUBMED    8386367
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-04-15"; volume: "90(8)"; pages: 
            "3216-20"
COMMENT     Sequence Label: pMyc2ElbLuc,
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(189..207)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(190..207)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(288..307)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     407..429
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(467..485)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        553..657
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(876..895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgggccttt ctttatgttt ttggcgtctt ccatttggat ccgggccctc tagatgcggc
       61 cgcatgcata agctaattcg agctcggtac cacattatat accctctgca ggcatgcaag
      121 cttagttgac cacgtggtca gttaagctta gttgaccacg tggtcagtta agcttcagct
      181 gctcgagttc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
      241 ttgtagccgc gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct
      301 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      361 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      421 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
      481 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      541 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      601 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      661 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      721 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      781 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      841 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      901 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      961 gagtactcac cagtc
//
