LOCUS       sequence-9264-ex        9829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 9829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC160
FEATURES             Location/Qualifiers
     source          1..9829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    60..184
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(209..214,215..232,233..239)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             293..973
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(359..378)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1293..1598
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1477..1496
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1639..1763)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1780..1796)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1781..1797)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(1852..1899)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1928..2605
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             2648..3322
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     protein_bind    complement(3821..3868)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3907..3927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3949..3969
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3991..4011
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4033..4053
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4096..4124)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4872..4892
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(5534..5545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7580..7684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7685..7753,7754..8545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7903..7922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8716..9304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9205..9224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9458..9475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9592..9613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9628..9633,9634..9651,9652..9658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9666..9682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9671..9693
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9690..9706
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9690..9706
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc tgatatcaaa
       61 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      121 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcct
      181 atgggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      241 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      301 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      361 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      421 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      481 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      541 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      601 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      661 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      721 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      781 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      841 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      901 aatgcttaat gaattacaac agtactgcga tgagtggcag gcggggcgta atctagagga
      961 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     1021 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     1081 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     1141 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     1201 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     1261 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     1321 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     1381 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac
     1441 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     1501 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     1561 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     1621 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     1681 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     1741 tcagctttct tgtacaaagt ggttcgatat caagcttatc gataccgtcg acctcgactc
     1801 tagaccgcgg ttttggcgcg gatccactag ttctagcttc aaaagcgctc tgaagttcct
     1861 atactttcta gagaatagga acttcggaat aggaacttca agatccccgg tagacgataa
     1921 ggatccgatg gtggcctcct ccgaggacgt catcaaagag ttcatgcgct tcaaggtgcg
     1981 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     2041 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     2101 ctgggacatc ctgtcccccc agttccagta cggctccaag gcgtacgtga agcaccccgc
     2161 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2221 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcac
     2281 gctgatctac aaggtgaagt tccgcggcac caacttcccc cccgacggcc ccgtaatgca
     2341 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     2401 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     2461 caagaccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacac
     2521 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     2581 cgagggccgc caccacctgt tcctggggca tggcaccggc agcaccggca gcggcagctc
     2641 cggcaccgcc tcctccgagg acgtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     2701 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2761 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     2821 catcctgtcc ccccagttcc agtacggctc caaggcgtac gtgaagcacc ccgccgacat
     2881 ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2941 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     3001 ctacaaggtg aagttccgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     3061 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     3121 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     3181 catctacatg gccaagaagc ccgtgcagct gcccggctac tactacgtgg acaccaagct
     3241 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     3301 ccgccaccac ctgttcctgt aggaattcgc tagccggcca tacaagtaat ccggatgatc
     3361 gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt
     3421 atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat
     3481 ctaaacatga gcggtttttt ttctctttcc gtctcccaat tcgtattccg ctcctctcat
     3541 ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg acgtgggggg
     3601 aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg acacacaata
     3661 tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat aaaaatatat
     3721 agaggaggaa ggaaaactag taaaaaataa gcaaagaaat taggcgaacg atgagaattg
     3781 tcctcgcttg ggcccaaaag gccttttttt tttggcctct gaagttccta tactttctag
     3841 agaataggaa cttcggaata ggaacttcaa gatccccggg taccgagctc agaaaaaatg
     3901 actgctccaa agaagaagcg taaggtaccg gcagaaaaaa tgactgctcc aaagaagaag
     3961 cgtaaggtac cggcagaaaa aatgactgct ccaaagaaga agcgtaaggt accggcagaa
     4021 aaaatgactg ctccaaagaa gaagcgtaag gtaccggtag aaaaaatgag taaaggagaa
     4081 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     4141 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     4201 tttatttgca ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta
     4261 actaaccctg attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt
     4321 caatgcttct cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
     4381 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     4441 cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa
     4501 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     4561 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     4621 atcatggcag acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta
     4681 actaactaat ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga
     4741 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     4801 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     4861 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggac
     4921 gaactataca aaagcccaca agctttggaa ttcgctagcc ggccatacaa gtaatccgga
     4981 tgatcgacgc caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc
     5041 ctattattta tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc
     5101 caaatctaaa catgagcggt tttttttctc tttccgtctc ccaattcgta ttccgctcct
     5161 ctcatctgaa cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg
     5221 gggggaattg gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca
     5281 caatatgcaa caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa
     5341 tatatagagg aggaaggaaa actagtctag aatcctatgc ttgcactttg agccaattta
     5401 tccaagtcct tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt attataatat
     5461 caaaaacaat ttgtcagttg accacttttt gatatatttg acagaaaccg ggatgaattg
     5521 gctcaaagtt agggcgccct tctattacag ggtttctgat aacaaaccgg ttattaactc
     5581 ccaacaaggg atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca
     5641 aattcatcgc ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt
     5701 ctgcacccta tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt
     5761 ctccccagct ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct
     5821 ctctgacgtc ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt
     5881 ttcccatttc tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt
     5941 ttttttctgt cggtgtgtgc tgctcggtta agagtgagct ctctattcca cgtcttcttc
     6001 tttttcttct tcgattcgaa tcaatcactc caccaaaacg cattcgtttt tgggattcac
     6061 cccgcggttc gcaacaggtt tctttttcaa attattagcg ttataaatag aaaaatggtg
     6121 gagtttcaat aaaaatgata atttacaaag tgtattttga ttacatgtac tcaaaaaggt
     6181 tgaaattttt cataccagtt ttccggaaat ccatctgata tcattatcgt atttttcttt
     6241 ttaaaaatgt tttcaaaaaa aaacaaaata tagctggtta tttggcaccc tctaattacc
     6301 attttcctgt cacaccacac tcttttcctt cttccacttc tttccgtttt cagccgcttc
     6361 caaccaaacc gatatgaaag ccgagcaaca acaatcgatt ccacccggct cggcgacctt
     6421 cccgtcgcag gtgagactca gaaaactaga gaaaccgctc aactaactct tgatatccga
     6481 tttcattctt ttctcttttc tttttgttga acttttccat atttccagat gccacggcca
     6541 ccaccaagca ccgaacaagg aatcacaacg gaatcggagc ttgcgaagaa agctcaaatc
     6601 actccgaacg acgttttagc acttccggga atcactcaag gtatcttgct ccctttggat
     6661 ttcagtttaa aacataaatt taggattctt atgctcccca tctgcgaaca tctataacat
     6721 cgagttcacc aagttccaaa tccgtgatct ggacactgag caagtgcttt tcgagatcgc
     6781 caaaccggag aacgatcagg agaatgatga gtcgccacag gagtcggcaa gatacgtgcg
     6841 ttatagattt gctccaaact ttttgaaact caaaacggtc ggagcaactg tggaattcaa
     6901 agtaggagac atcccaatcc atcatttccg aatgatcgaa cgtcacttct tcaaagatcg
     6961 ccttctgaag tgttttgact ttgaattcgg attctgtatt ccgaattcac gaaacaactg
     7021 tgaacatatc tatgagttcc ctcaactctc tcaacaactc agtgagtcat tattctaaaa
     7081 agtacaattc aaaagactaa tctctttttc agtggacgac atgatcaaca atccaaacga
     7141 gactcgttct gacagcttct atttcgtcga taacaaactc gtcatgcaca acaaagccga
     7201 ctactcatat gatgcataaa tatttaatac aaaaatgttc tggataatta ttctgtcgaa
     7261 tagaaaaaaa actccaaatg tgattaaatt ccaataattc ctgtctagtt tgttccttcc
     7321 ccttcccctt ctcatgttca atgcattcct aagcttttca gttcccccct tgtttctata
     7381 ttttttcgct gtcctgtcac actcgctaaa aacactaatc acacggaaat ctgttttcaa
     7441 taaaaactcc aactttaact cattttcaat ttcaactgaa agattttttc attagagaat
     7501 gtctaggcat gctctagagg gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg
     7561 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7621 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7681 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7741 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7801 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7861 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7921 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7981 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8041 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8101 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8161 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8221 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8281 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8341 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8401 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8461 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8521 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8581 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8641 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8701 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8761 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8821 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     8881 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8941 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9001 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9061 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     9121 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9181 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9241 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9301 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9361 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9421 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9481 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9541 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9601 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9661 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     9721 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
     9781 aaaatggctg aaatcactca caacgatgga tacgctaaca acttggaaa
//
