LOCUS       sequence-9265-ex       10838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC162
FEATURES             Location/Qualifiers
     source          1..10838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     443..459
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     444..460
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    475..599
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(624..629,630..647,648..654)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             708..1367
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1709..2014
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2055..2179)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2218..2265)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2304..2324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2346..2366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2388..2408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2430..2450
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2493..2521)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3269..3289
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(3931..3942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5975..6022)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    6060..6183
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6224..6529)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(6871..7530)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(join(7584..7590,7591..7608,7609..7614))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(7640..7763)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8278..8300)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8400..8419
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8497..8515)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8583..8687
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8688..8756,8757..9548)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8906..8925)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9719..10307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10208..10227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10461..10478
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10595..10616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10631..10636,10637..10654,10655..10661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10669..10685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10674..10696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10693..10709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10693..10709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagtttgt gcaacaaatt gaggaaggca ttctagagaa gaatttcggc
       61 atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa ccggtttgaa
      121 ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac aataaattca
      181 caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata catctagaat
      241 cctttgctgt agaagaattg ctcaataaga acacgtggag ctcctttaat tttgatacga
      301 cctttcaact catcgacgtg actggcgatc gaatgtccaa gatgttcttc aagataggag
      361 cggagatcat tttcgcaagc ctgaaagata tttatgttga ttcaggattt aataatgttc
      421 ccaacttaca aaaatgtctc cgtcgaggtc gacggtatcg ataagcttga tatcacaagt
      481 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
      541 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcatattgg
      601 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
      661 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      721 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      781 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      841 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      901 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
      961 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1021 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1081 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1141 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1201 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1261 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1321 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg
     1381 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     1441 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     1501 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     1561 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     1621 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     1681 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     1741 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     1801 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     1861 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     1921 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     1981 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     2041 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagcattat gtagtctgtt
     2101 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     2161 ctttcttgta caaagtggtg atatcagatc cactagttct agcttcaaaa gcgctctgaa
     2221 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat ccccgggtac
     2281 cgagctcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggca gaaaaaatga
     2341 ctgctccaaa gaagaagcgt aaggtaccgg cagaaaaaat gactgctcca aagaagaagc
     2401 gtaaggtacc ggcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggtagaaa
     2461 aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     2521 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     2581 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tgggtaagtt
     2641 taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca cttgtcacta
     2701 ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa cggcatgact
     2761 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     2821 acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca tacatattta
     2881 aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     2941 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     3001 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt
     3061 taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc aaaattagac
     3121 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     3181 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     3241 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     3301 ttacacatgg catggacgaa ctatacaaaa gcccacaagc tttggaattc gctagccggc
     3361 catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg
     3421 aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct
     3481 aaaaaattaa ttcaatccaa atctaaacat gagcggtttt ttttctcttt ccgtctccca
     3541 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     3601 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     3661 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     3721 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtctagaat cctatgcttg
     3781 cactttgagc caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa
     3841 aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca
     3901 gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac
     3961 aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca
     4021 aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta
     4081 ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc
     4141 ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt
     4201 ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct
     4261 tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac
     4321 cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc
     4381 tattccacgt cttcttcttt ttcttcttcg attcgaatca atcactccac caaaacgcat
     4441 tcgtttttgg gattcacccc gcggttcgca acaggtttct ttttcaaatt attagcgtta
     4501 taaatagaaa aatggtggag tttcaataaa aatgataatt tacaaagtgt attttgatta
     4561 catgtactca aaaaggttga aatttttcat accagttttc cggaaatcca tctgatatca
     4621 ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa caaaatatag ctggttattt
     4681 ggcaccctct aattaccatt ttcctgtcac accacactct tttccttctt ccacttcttt
     4741 ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg agcaacaaca atcgattcca
     4801 cccggctcgg cgaccttccc gtcgcaggtg agactcagaa aactagagaa accgctcaac
     4861 taactcttga tatccgattt cattcttttc tcttttcttt ttgttgaact tttccatatt
     4921 tccagatgcc acggccacca ccaagcaccg aacaaggaat cacaacggaa tcggagcttg
     4981 cgaagaaagc tcaaatcact ccgaacgacg ttttagcact tccgggaatc actcaaggta
     5041 tcttgctccc tttggatttc agtttaaaac ataaatttag gattcttatg ctccccatct
     5101 gcgaacatct ataacatcga gttcaccaag ttccaaatcc gtgatctgga cactgagcaa
     5161 gtgcttttcg agatcgccaa accggagaac gatcaggaga atgatgagtc gccacaggag
     5221 tcggcaagat acgtgcgtta tagatttgct ccaaactttt tgaaactcaa aacggtcgga
     5281 gcaactgtgg aattcaaagt aggagacatc ccaatccatc atttccgaat gatcgaacgt
     5341 cacttcttca aagatcgcct tctgaagtgt tttgactttg aattcggatt ctgtattccg
     5401 aattcacgaa acaactgtga acatatctat gagttccctc aactctctca acaactcagt
     5461 gagtcattat tctaaaaagt acaattcaaa agactaatct ctttttcagt ggacgacatg
     5521 atcaacaatc caaacgagac tcgttctgac agcttctatt tcgtcgataa caaactcgtc
     5581 atgcacaaca aagccgacta ctcatatgat gcataaatat ttaatacaaa aatgttctgg
     5641 ataattattc tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca ataattcctg
     5701 tctagtttgt tccttcccct tccccttctc atgttcaatg cattcctaag cttttcagtt
     5761 ccccccttgt ttctatattt tttcgctgtc ctgtcacact cgctaaaaac actaatcaca
     5821 cggaaatctg ttttcaataa aaactccaac tttaactcat tttcaatttc aactgaaaga
     5881 ttttttcatt agagaatgtc taggcatgcc tgcaggtcga ctctagaccg cggttttggc
     5941 gcggatccac tagttctagc ttcaaaagcg ctctgaagtt cctatacttt ctagagaata
     6001 ggaacttcgg aataggaact tcaagatccc cgggtaccga gctcattacg ccaagctatc
     6061 aactttgtat agaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     6121 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     6181 atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
     6241 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
     6301 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
     6361 cgatatgcac caccgggtaa agttcacggg ggactttatc tgacagcaga cgtgcactgg
     6421 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
     6481 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
     6541 ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
     6601 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
     6661 gtgcttaccg aaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
     6721 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
     6781 atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta
     6841 tctggctttt agtaagccgg atcctctaga ttacgccccg ccctgccact catcgcagta
     6901 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     6961 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     7021 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     7081 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     7141 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     7201 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     7261 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     7321 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     7381 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     7441 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     7501 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     7561 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
     7621 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
     7681 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     7741 ttcaacttta ttatacatag ttgataattc actgggctag ccggccatac aagtaatccg
     7801 gatgatcgac gccaacgtcg ttgaattttc aaattttaaa tactgaatat ttgttttttt
     7861 tcctattatt tatttattct ctttgtgttt tttttcttgc tttctaaaaa attaattcaa
     7921 tccaaatcta aacatgagcg gttttttttc tctttccgtc tcccaattcg tattccgctc
     7981 ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg
     8041 tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca
     8101 cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa
     8161 aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg
     8221 agaattgtcc tcgcttgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     8281 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8341 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     8401 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     8461 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
     8521 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     8581 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8641 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8701 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8761 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8821 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8881 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8941 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9001 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9061 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9121 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9181 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9241 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9301 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9361 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9421 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9481 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9541 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9601 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9661 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9721 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9781 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9841 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     9901 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9961 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10021 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10081 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
    10141 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10201 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10261 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10321 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10381 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10441 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10501 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10561 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10621 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10681 aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat
    10741 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
    10801 tcacaacgat ggatacgcta acaacttgga aatgaaat
//
