LOCUS       sequence-92651-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta-glucosidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Carlin DA, Caster RW, Wang X, Betzenderfer SA, Chen CX, Duong VM, 
            Ryklansky CV, Alpekin A, Beaumont N, Kapoor H, Kim N, Mohabbot H, 
            Pang B, Teel R, Whithaus L, Tagkopoulos I, Siegel JB
  TITLE     Kinetic Characterization of 100 Glycoside Hydrolase Mutants Enables 
            the Discovery of Structural Features Correlated with Kinetic 
            Constants.
  JOURNAL   PLoS One. 2016 Jan 27;11(1):e0147596. doi: 
            10.1371/journal.pone.0147596. eCollection 2016.
  PUBMED    26815142
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-27"; volume: "11(1):e0147596. doi"; pages: " 
            10.1371/journal.pone.0147596. eCollection 2016"
COMMENT     Sequence Label: BglB in pET29b(+)
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..54)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcttccttt cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag
       61 gctgccgaaa ccatttttgg ccatcatttg tttaaaccac agtgcgctct gtttcggggt
      121 gcgttcttgg gtttcatagt tgatgtggac aataccaaaa cgtttgctat aaccccatgc
      181 ccattcaaag ttatccagaa agctccaaac aaaataacct ttcagctggc caccttcttc
      241 gataaagcgg tggcaggctt tcagatgttc ttcaatataa ccatgacgac cggtatcttc
      301 gatctgacca ttaaccagtt catcacgcat tgctgcacca ttttcggtaa tcaggatcgg
      361 cagacctttg ctaaaatctt tttcaatgcg ggtcagcagt ttatagaagc tttccggatg
      421 aatttcccaa cccatatcgg taaccggttc ttccatgtga acctgttcaa cttgcagcag
      481 gcttgcatca ttggtgctgc gaataatgct acgggtataa tagttaatgc ccagaaaatc
      541 acctggctgc tgaatcagtt ccatatcacc aggctgaaca aaatccagac cattcagata
      601 ggtgccatac cattccacca tatcttcagg atatttacca ttgaacagcg gttctgcaaa
      661 ccaacgattg ataaaaccat cacgacgaat tgctgcggca acatcttccg gacgttcgct
      721 tgctgcatca acatgttcca tattcagggt aatgccaatt ttaccggtca gacctttttc
      781 tttatgcagg ttgcttgcaa taccatgaca catcagaata tgatgtgctg cggtaaatgc
      841 ttcacgccaa ttttcatgac ccggtgcatg ttcaccggtg ccataaccca gaatgcttgc
      901 acaatacggt tcattgatgg tgttccacca attaatacgt tcaccaaaac gatccatgat
      961 aacgctggca taggttt
//
