LOCUS       sequence-92653-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Song H, Payne S, Gray M, You L
  TITLE     Spatiotemporal modulation of biodiversity in a synthetic 
            chemical-mediated ecosystem.
  JOURNAL   Nat Chem Biol. 2009 Dec;5(12):929-35. Epub 2009 Nov 1.
  PUBMED    19915540
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-12"; volume: "5(12)"; pages: "929-35. Epub 2009 
            Nov 1"
COMMENT     Sequence Label: ptetmCherry_prey_plasmid-2
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(31..133)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     199..217
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(238..256,257..262,263..279,280..285,286..311)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    238..256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    263..281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             321..332
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(484..502)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     752..771
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
       61 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
      121 tacgtgccga tcaacgtctc attttcgcca gatatcgacg tctaagaaac cattattatc
      181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagtcc
      241 ctatcagtga tagagattga catccctatc agtgatagag atactgagca catcagcagg
      301 acgcactgac cgaattcatt aaagaggaga aaggtaccat ggtgagcaag ggcgaggagg
      361 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      421 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      481 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      541 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      601 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      661 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      721 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      781 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      841 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      901 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      961 cccacaacga ggactacacc atc
//
