LOCUS       sequence-92657-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA against alpha-actinin-2 with eGFP transfection marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Schnizler MK, Schnizler K, Zha XM, Hall DD, Wemmie JA, Hell JW, 
            Welsh MJ
  TITLE     The cytoskeletal protein alpha-actinin regulates acid-sensing ion 
            channel 1a through a C-terminal interaction.
  JOURNAL   J Biol Chem. 2009 Jan 30;284(5):2697-705. doi: 
            10.1074/jbc.M805110200. Epub 2008 Nov 21.
  PUBMED    19028690
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M805110200"; journalName: "J Biol Chem"; date: 
            "2009-01-30- 30"; volume: "284"; issue: "5"; pages: "2697-705"
COMMENT     Sequence Label: pSIL-eGFP-shACTN2
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..65
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(143..456)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(204..224)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(453..469)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(526..547)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     875..894
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt
       61 ggatcccccg ggctgcagga attaaaaaat ggcctgatgg atcatgagtc tcttgaactc
      121 atgatccatc aggccaggcc caaacaaggc ttttctccaa gggatattta tagtctcaaa
      181 acacacaatt actttacagt tagggtgagt ttccttttgt gctgtttttt aaaataataa
      241 tttagtattt gtatctctta tagaaatcca agcctatcat gtaaaatgta gctagtatta
      301 aaaagaacag attatctgtc ttttatcgca cattaagcct ctatagttac taggaaatat
      361 tatatgcaaa ttaaccgggg caggggagta gccgagcttc tcccacaagt ctgtgcgagg
      421 gggccggcgc gggcctagag atggcggcgt cggatccact agttctagag cgggtaccta
      481 gtacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      541 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
      601 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      661 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
      721 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      781 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      841 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      901 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      961 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggt
//
