LOCUS       sequence-92658-e         366 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA against alpha-actinin-2 with eGFP transfection marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Schnizler MK, Schnizler K, Zha XM, Hall DD, Wemmie JA, Hell JW, 
            Welsh MJ
  TITLE     The cytoskeletal protein alpha-actinin regulates acid-sensing ion 
            channel 1a through a C-terminal interaction.
  JOURNAL   J Biol Chem. 2009 Jan 30;284(5):2697-705. doi: 
            10.1074/jbc.M805110200. Epub 2008 Nov 21.
  PUBMED    19028690
REFERENCE   2  (bases 1 to 366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M805110200"; journalName: "J Biol Chem"; date: 
            "2009-01-30- 30"; volume: "284"; issue: "5"; pages: "2697-705"
COMMENT     Sequence Label: pSIL-eGFP-shACTN2
FEATURES             Location/Qualifiers
     source          1..366
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..52
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     36..52
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        49..362
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     281..301
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ggcatggacg agctgtacaa gtactaggta cccgctctag aactagtgga tccgacgccg
       61 ccatctctag gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc
      121 cctgccccgg ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat
      181 aaaagacaga taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt
      241 tctataagag atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac
      301 cctaactgta aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg
      361 tttggg
//
