LOCUS       sequence-92659-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta-glucosidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Carlin DA, Caster RW, Wang X, Betzenderfer SA, Chen CX, Duong VM, 
            Ryklansky CV, Alpekin A, Beaumont N, Kapoor H, Kim N, Mohabbot H, 
            Pang B, Teel R, Whithaus L, Tagkopoulos I, Siegel JB
  TITLE     Kinetic Characterization of 100 Glycoside Hydrolase Mutants Enables 
            the Discovery of Structural Features Correlated with Kinetic 
            Constants.
  JOURNAL   PLoS One. 2016 Jan 27;11(1):e0147596. doi: 
            10.1371/journal.pone.0147596. eCollection 2016.
  PUBMED    26815142
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-27"; volume: "11(1):e0147596. doi"; pages: " 
            10.1371/journal.pone.0147596. eCollection 2016"
COMMENT     Sequence Label: BglB in pET29b(+)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..50
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(103..120)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
       61 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      121 ctcgaggctg ccgaaaccat ttttggccat catttgttta aaccacagtg cgctctgttt
      181 cggggtgcgt tcttgggttt catagttgat gtggacaata ccaaaacgtt tgctataacc
      241 ccatgcccat tcaaagttat ccagaaagct ccaaacaaaa taacctttca gctggccacc
      301 ttcttcgata aagcggtggc aggctttcag atgttcttca atataaccat gacgaccggt
      361 atcttcgatc tgaccattaa ccagttcatc acgcattgct gcaccatttt cggtaatcag
      421 gatcggcaga cctttgctaa aatctttttc aatgcgggtc agcagtttat agaagctttc
      481 cggatgaatt tcccaaccca tatcggtaac cggttcttcc atgtgaacct gttcaacttg
      541 cagcaggctt gcatcattgg tgctgcgaat aatgctacgg gtataatagt taatgcccag
      601 aaaatcacct ggctgctgaa tcagttccat atcaccaggc tgaacaaaat ccagaccatt
      661 cagataggtg ccataccatt ccaccatatc ttcaggatat ttaccattga acagcggttc
      721 tgcaaaccaa cgattgataa aaccatcacg acgaattgct gcggcaacat cttccggacg
      781 ttcgcttgct gcatcaacat gttccatatt cagggtaatg ccaattttac cggtcagacc
      841 tttt
//
