LOCUS       sequence-9267-ex       10795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC163
FEATURES             Location/Qualifiers
     source          1..10795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    60..184
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(209..214,215..232,233..239)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             293..973
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             1293..1598
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1639..1763)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1780..1796)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1781..1797)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(1852..1899)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1928..2605
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             2648..3322
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     protein_bind    complement(3821..3868)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3906..4029
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4070..4375)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(4717..5376)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(join(5430..5436,5437..5454,5455..5460))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5486..5609)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6124..6146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6246..6265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6343..6361)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(6500..6511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8546..8650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8651..8719,8720..9511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8869..8888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9682..10270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10171..10190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10424..10441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10558..10579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10594..10599,10600..10617,10618..10624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10632..10648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10637..10659
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10656..10672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10656..10672
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc tgatatcaaa
       61 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      121 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcct
      181 atgggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      241 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      301 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      361 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      421 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      481 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      541 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      601 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      661 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      721 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      781 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      841 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      901 aatgcttaat gaattacaac agtactgcga tgagtggcag gcggggcgta atctagagga
      961 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     1021 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     1081 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     1141 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     1201 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     1261 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     1321 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     1381 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac
     1441 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     1501 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     1561 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     1621 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     1681 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     1741 tcagctttct tgtacaaagt ggttcgatat caagcttatc gataccgtcg acctcgactc
     1801 tagaccgcgg ttttggcgcg gatccactag ttctagcttc aaaagcgctc tgaagttcct
     1861 atactttcta gagaatagga acttcggaat aggaacttca agatccccgg tagacgataa
     1921 ggatccgatg gtggcctcct ccgaggacgt catcaaagag ttcatgcgct tcaaggtgcg
     1981 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     2041 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     2101 ctgggacatc ctgtcccccc agttccagta cggctccaag gcgtacgtga agcaccccgc
     2161 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2221 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcac
     2281 gctgatctac aaggtgaagt tccgcggcac caacttcccc cccgacggcc ccgtaatgca
     2341 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     2401 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     2461 caagaccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacac
     2521 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     2581 cgagggccgc caccacctgt tcctggggca tggcaccggc agcaccggca gcggcagctc
     2641 cggcaccgcc tcctccgagg acgtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     2701 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2761 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     2821 catcctgtcc ccccagttcc agtacggctc caaggcgtac gtgaagcacc ccgccgacat
     2881 ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2941 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     3001 ctacaaggtg aagttccgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     3061 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     3121 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     3181 catctacatg gccaagaagc ccgtgcagct gcccggctac tactacgtgg acaccaagct
     3241 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     3301 ccgccaccac ctgttcctgt aggaattcgc tagccggcca tacaagtaat ccggatgatc
     3361 gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt
     3421 atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat
     3481 ctaaacatga gcggtttttt ttctctttcc gtctcccaat tcgtattccg ctcctctcat
     3541 ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg acgtgggggg
     3601 aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg acacacaata
     3661 tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat aaaaatatat
     3721 agaggaggaa ggaaaactag taaaaaataa gcaaagaaat taggcgaacg atgagaattg
     3781 tcctcgcttg ggcccaaaag gccttttttt tttggcctct gaagttccta tactttctag
     3841 agaataggaa cttcggaata ggaacttcaa gatccccggg taccgagctc attacgccaa
     3901 gctatcaact ttgtatagaa aagttgaacg agaaacgtaa aatgatataa atatcaatat
     3961 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     4021 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
     4081 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     4141 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     4201 catccccgat atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg
     4261 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     4321 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     4381 cagcccctgt tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     4441 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     4501 atggttgtgc ttaccgaacc ggagatattg acatcatata tgccttgagc aactgatagc
     4561 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
     4621 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
     4681 ctgttatctg gcttttagta agccggatcc tctagattac gccccgccct gccactcatc
     4741 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     4801 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     4861 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     4921 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     4981 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     5041 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     5101 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     5161 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     5221 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     5281 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     5341 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     5401 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
     5461 gcctgggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
     5521 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     5581 ttctcgttca actttattat acatagttga taattcactg ggctagccgg ccatacaagt
     5641 aatccggatg atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt
     5701 tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta
     5761 attcaatcca aatctaaaca tgagcggttt tttttctctt tccgtctccc aattcgtatt
     5821 ccgctcctct catctgaaca caatgtgcaa gtttatttat cttctcgctt tcatttcatt
     5881 aggacgtggg gggaattggt ggaaggggga aacacacaaa aggatgatgg aaatgaaata
     5941 aggacacaca atatgcaaca acattcaatt cagaaatatg gaggaaggtt taaaagaaaa
     6001 cataaaaata tatagaggag gaaggaaaac tagtaaaaaa taagcaaaga aattaggcga
     6061 acgatgagaa ttgtcctcgc ttgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6301 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     6361 ttttataggt taatgtcatg ataataatgg tttcttagac aacggataaa atgtttatta
     6421 taatatcaaa aacaatttgt cagttgacca ctttttgata tatttgacag aaaccgggat
     6481 gaattggctc aaagttaggg cgcccttcta ttacagggtt tctgataaca aaccggttat
     6541 taactcccaa caagggatga tttcaattca tcatgctcaa attgacccaa attaagttac
     6601 atgacaaatt catcgccctt ttcaactctt cttggctcat catctgttat tctgttccta
     6661 ttctgtctgc accctatacc cctttgcata ctctctcgtc attcctttct tggatagtgt
     6721 cttcttctcc ccagctctgc tacttctatg acttgcgccc gctgcttttc cgctcgttcc
     6781 tctcctctct gacgtcttct tctgcttctt ttctccttcc tttttccctt ctcaatcttc
     6841 ctctttttcc catttctgtc aatcattcga agaagaagaa gaagaagacc ctcattcatt
     6901 tcattttttt ttctgtcggt gtgtgctgct cggttaagag tgagctctct attccacgtc
     6961 ttcttctttt tcttcttcga ttcgaatcaa tcactccacc aaaacgcatt cgtttttggg
     7021 attcaccccg cggttcgcaa caggtttctt tttcaaatta ttagcgttat aaatagaaaa
     7081 atggtggagt ttcaataaaa atgataattt acaaagtgta ttttgattac atgtactcaa
     7141 aaaggttgaa atttttcata ccagttttcc ggaaatccat ctgatatcat tatcgtattt
     7201 ttctttttaa aaatgttttc aaaaaaaaac aaaatatagc tggttatttg gcaccctcta
     7261 attaccattt tcctgtcaca ccacactctt ttccttcttc cacttctttc cgttttcagc
     7321 cgcttccaac caaaccgata tgaaagccga gcaacaacaa tcgattccac ccggctcggc
     7381 gaccttcccg tcgcaggtga gactcagaaa actagagaaa ccgctcaact aactcttgat
     7441 atccgatttc attcttttct cttttctttt tgttgaactt ttccatattt ccagatgcca
     7501 cggccaccac caagcaccga acaaggaatc acaacggaat cggagcttgc gaagaaagct
     7561 caaatcactc cgaacgacgt tttagcactt ccgggaatca ctcaaggtat cttgctccct
     7621 ttggatttca gtttaaaaca taaatttagg attcttatgc tccccatctg cgaacatcta
     7681 taacatcgag ttcaccaagt tccaaatccg tgatctggac actgagcaag tgcttttcga
     7741 gatcgccaaa ccggagaacg atcaggagaa tgatgagtcg ccacaggagt cggcaagata
     7801 cgtgcgttat agatttgctc caaacttttt gaaactcaaa acggtcggag caactgtgga
     7861 attcaaagta ggagacatcc caatccatca tttccgaatg atcgaacgtc acttcttcaa
     7921 agatcgcctt ctgaagtgtt ttgactttga attcggattc tgtattccga attcacgaaa
     7981 caactgtgaa catatctatg agttccctca actctctcaa caactcagtg agtcattatt
     8041 ctaaaaagta caattcaaaa gactaatctc tttttcagtg gacgacatga tcaacaatcc
     8101 aaacgagact cgttctgaca gcttctattt cgtcgataac aaactcgtca tgcacaacaa
     8161 agccgactac tcatatgatg cataaatatt taatacaaaa atgttctgga taattattct
     8221 gtcgaataga aaaaaaactc caaatgtgat taaattccaa taattcctgt ctagtttgtt
     8281 ccttcccctt ccccttctca tgttcaatgc attcctaagc ttttcagttc cccccttgtt
     8341 tctatatttt ttcgctgtcc tgtcacactc gctaaaaaca ctaatcacac ggaaatctgt
     8401 tttcaataaa aactccaact ttaactcatt ttcaatttca actgaaagat tttttcatta
     8461 gagaatgtct aggcatgctc tagagggccc aaaaggcctt ttttttttgg cgccgacgtc
     8521 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8581 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8641 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8701 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8761 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8821 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8881 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8941 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9001 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9061 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9121 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9181 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9241 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9301 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9361 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9421 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9481 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9541 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9601 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9661 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9721 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9781 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     9841 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9901 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9961 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10021 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
    10081 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10141 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10201 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10261 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10321 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10381 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10441 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10501 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    10561 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    10621 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    10681 cgccaagctg taagtttaaa catgatctta ctaactaact attctcattt aaattttcag
    10741 agcttaaaaa tggctgaaat cactcacaac gatggatacg ctaacaactt ggaaa
//
