LOCUS       sequence-92671-e        1986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zeocin-resistance plasmid for insertional mutagenesis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Mukaiyama H, Oku M, Baba M, Samizo T, Hammond AT, Glick BS, Kato N, 
            Sakai Y
  TITLE     Paz2 and 13 other PAZ gene products regulate vacuolar engulfment of 
            peroxisomes during micropexophagy.
  JOURNAL   Genes Cells. 2002 Jan;7(1):75-90.
  PUBMED    11856375
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells."; date: "2002-01"; volume: "7(1)"; pages: "75-90"
COMMENT     Sequence Label: pREMI-Z
FEATURES             Location/Qualifiers
     source          1..1986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        70..483
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        490..537
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             556..930
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      996..1243
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1012..1030)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(1318..1906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1398..1417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatcgtaaa acgacggcca gtctagctag ctaggatcct gactgactga catggtcata
       61 gctgtttccg atcccccaca caccatagct tcaaaatgtt tctactcctt ttttactctt
      121 ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa gcacagcata
      181 ctaaatttcc cctctttctt cctctagggt gtcgttaatt acccgtacta aaggtttgga
      241 aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt
      301 atcacgtttc tttttcttga aaattttttt ttttgatttt tttctctttc gatgacctcc
      361 cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt catttttctt
      421 gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc aatctaatct
      481 aagggcggtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
      541 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
      601 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
      661 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
      721 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
      781 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
      841 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
      901 gtgcacttcg tggccgagga gcaggactga cacgtccgac gcggcccgac gggtccgagg
      961 cctcggagat ccgtccccct tttcctttgt cgatatcatg taattagtta tgtcacgctt
     1021 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
     1081 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
     1141 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
     1201 cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcaagctgg agaccaacat
     1261 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1321 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1381 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1441 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1501 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1561 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1621 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1681 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1741 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1801 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1981 gagatc
//
