LOCUS       sequence-9268-ex       10407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC225
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    60..184
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(209..214,215..232,233..239)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             293..952
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1294..1599
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1640..1764)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1780..1796)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1781..1797)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(1852..1899)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1938..1958
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1980..2000
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2022..2042
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2064..2084
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2106..2783
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             2826..3500
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     protein_bind    complement(3776..3823)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3861..3984
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4025..4330)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(4672..5331)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(join(5385..5391,5392..5409,5410..5415))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5441..5564)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6157..6168)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8323..8344
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8507..8611
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8612..8680,8681..9472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8830..8849)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9643..10231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10132..10151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10385..10402
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatatcaa
       61 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      121 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcat
      181 attggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      241 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      301 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      361 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      421 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      481 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      541 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      601 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      661 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      721 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      781 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      841 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      901 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg
      961 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     1021 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     1081 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     1141 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     1201 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     1261 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     1321 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     1381 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     1441 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     1501 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     1561 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     1621 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     1681 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     1741 ttcagctttc ttgtacaaag tggttgatat caagcttatc gataccgtcg acctcgactc
     1801 tagaccgcgg ttttggcgcg gatccactag ttctagcttc aaaagcgctc tgaagttcct
     1861 atactttcta gagaatagga acttcggaat aggaacttca agatccccgg gtaccgagct
     1921 cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggcagaaaaa atgactgctc
     1981 caaagaagaa gcgtaaggta ccggcagaaa aaatgactgc tccaaagaag aagcgtaagg
     2041 taccggcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggta gacgataagg
     2101 atccgatggt ggcctcctcc gaggacgtca tcaaagagtt catgcgcttc aaggtgcgca
     2161 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     2221 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     2281 gggacatcct gtccccccag ttccagtacg gctccaaggc gtacgtgaag caccccgccg
     2341 acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     2401 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     2461 tgatctacaa ggtgaagttc cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     2521 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     2581 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     2641 agaccatcta catggccaag aagcccgtgc agctgcccgg ctactactac gtggacacca
     2701 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     2761 agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg
     2821 gcaccgcctc ctccgaggac gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg
     2881 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     2941 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     3001 tcctgtcccc ccagttccag tacggctcca aggcgtacgt gaagcacccc gccgacatcc
     3061 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3121 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct
     3181 acaaggtgaa gttccgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga
     3241 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     3301 agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca
     3361 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac accaagctgg
     3421 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc
     3481 gccaccacct gttcctgtag gaattcgcta gccggccata caagtaatcc ggatgatcga
     3541 cgccaacgtc gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat
     3601 ttatttattc tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct
     3661 aaacatgagc ggtttttttt ctctttccgt ctcccaattc gtattccgct cctctcatct
     3721 gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac gctctgaagt
     3781 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaagatcc ccgggtaccg
     3841 agctcattac gccaagctat caactttgta tagaaaagtt gaacgagaaa cgtaaaatga
     3901 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     3961 aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata agggagcctg
     4021 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
     4081 tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca
     4141 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gggactttat
     4201 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
     4261 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
     4321 taaactgcat ttcaccagcc cctgttctcg tcggcaaaag agccgttcat ttcaataaac
     4381 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
     4441 cgggcttcat tctgcatggt tgtgcttacc gaaccggaga tattgacatc atatatgcct
     4501 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
     4561 tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc
     4621 gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatcctctag attacgcccc
     4681 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     4741 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     4801 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     4861 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     4921 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     4981 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     5041 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     5101 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     5161 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     5221 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     5281 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     5341 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
     5401 agcataaagt gtaaagcctg ggggtgccta atgcggccgc catagtgact ggatatgttg
     5461 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     5521 tatatcattt tacgtttctc gttcaacttt attatacata gttgataatt cactgggcta
     5581 gccggccata caagtaatcc ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     5641 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     5701 ctttctaaaa aattaattca atccaaatct aaacatgagc ggtttttttt ctctttccgt
     5761 ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct
     5821 cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat
     5881 gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa atatggagga
     5941 aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaaactagtc tagaatccta
     6001 tgcttgcact ttgagccaat ttatccaagt ccttgtaaaa agtattcgaa aattgttaac
     6061 ggataaaatg tttattataa tatcaaaaac aatttgtcag ttgaccactt tttgatatat
     6121 ttgacagaaa ccgggatgaa ttggctcaaa gttagggcgc ccttctatta cagggtttct
     6181 gataacaaac cggttattaa ctcccaacaa gggatgattt caattcatca tgctcaaatt
     6241 gacccaaatt aagttacatg acaaattcat cgcccttttc aactcttctt ggctcatcat
     6301 ctgttattct gttcctattc tgtctgcacc ctatacccct ttgcatactc tctcgtcatt
     6361 cctttcttgg atagtgtctt cttctcccca gctctgctac ttctatgact tgcgcccgct
     6421 gcttttccgc tcgttcctct cctctctgac gtcttcttct gcttcttttc tccttccttt
     6481 ttcccttctc aatcttcctc tttttcccat ttctgtcaat cattcgaaga agaagaagaa
     6541 gaagaccctc attcatttca tttttttttc tgtcggtgtg tgctgctcgg ttaagagtga
     6601 gctctctatt ccacgtcttc ttctttttct tcttcgattc gaatcaatca ctccaccaaa
     6661 acgcattcgt ttttgggatt caccccgcgg ttcgcaacag gtttcttttt caaattatta
     6721 gcgttataaa tagaaaaatg gtggagtttc aataaaaatg ataatttaca aagtgtattt
     6781 tgattacatg tactcaaaaa ggttgaaatt tttcatacca gttttccgga aatccatctg
     6841 atatcattat cgtatttttc tttttaaaaa tgttttcaaa aaaaaacaaa atatagctgg
     6901 ttatttggca ccctctaatt accattttcc tgtcacacca cactcttttc cttcttccac
     6961 ttctttccgt tttcagccgc ttccaaccaa accgatatga aagccgagca acaacaatcg
     7021 attccacccg gctcggcgac cttcccgtcg caggtgagac tcagaaaact agagaaaccg
     7081 ctcaactaac tcttgatatc cgatttcatt cttttctctt ttctttttgt tgaacttttc
     7141 catatttcca gatgccacgg ccaccaccaa gcaccgaaca aggaatcaca acggaatcgg
     7201 agcttgcgaa gaaagctcaa atcactccga acgacgtttt agcacttccg ggaatcactc
     7261 aaggtatctt gctccctttg gatttcagtt taaaacataa atttaggatt cttatgctcc
     7321 ccatctgcga acatctataa catcgagttc accaagttcc aaatccgtga tctggacact
     7381 gagcaagtgc ttttcgagat cgccaaaccg gagaacgatc aggagaatga tgagtcgcca
     7441 caggagtcgg caagatacgt gcgttataga tttgctccaa actttttgaa actcaaaacg
     7501 gtcggagcaa ctgtggaatt caaagtagga gacatcccaa tccatcattt ccgaatgatc
     7561 gaacgtcact tcttcaaaga tcgccttctg aagtgttttg actttgaatt cggattctgt
     7621 attccgaatt cacgaaacaa ctgtgaacat atctatgagt tccctcaact ctctcaacaa
     7681 ctcagtgagt cattattcta aaaagtacaa ttcaaaagac taatctcttt ttcagtggac
     7741 gacatgatca acaatccaaa cgagactcgt tctgacagct tctatttcgt cgataacaaa
     7801 ctcgtcatgc acaacaaagc cgactactca tatgatgcat aaatatttaa tacaaaaatg
     7861 ttctggataa ttattctgtc gaatagaaaa aaaactccaa atgtgattaa attccaataa
     7921 ttcctgtcta gtttgttcct tccccttccc cttctcatgt tcaatgcatt cctaagcttt
     7981 tcagttcccc ccttgtttct atattttttc gctgtcctgt cacactcgct aaaaacacta
     8041 atcacacgga aatctgtttt caataaaaac tccaacttta actcattttc aatttcaact
     8101 gaaagatttt ttcattagag aatgtctagg catgctctag agggcccaaa aggggctttc
     8161 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8221 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8281 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8341 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8401 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     8461 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8581 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8641 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8701 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8761 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8821 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8881 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8941 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9001 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9061 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9121 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9181 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9241 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9301 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9361 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9421 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9481 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9541 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9601 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9661 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9721 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9781 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9841 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9901 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9961 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10021 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10081 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10141 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10261 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    10321 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    10381 gcgcagcgag tcagtgagcg aggaagc
//
