LOCUS       sequence-92683-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses halo tagged PTEN protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Fang X, Fu Y, Long MJ, Haegele JA, Ge EJ, Parvez S, Aye Y
  TITLE     Temporally controlled targeting of 4-hydroxynonenal to specific 
            proteins in living cells.
  JOURNAL   J Am Chem Soc. 2013 Oct 2;135(39):14496-9. doi: 10.1021/ja405400k. 
            Epub 2013 Sep 18.
  PUBMED    24015839
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja405400k";
            journalName: "J Am Chem Soc"; date: "2013-10-2- 2"; volume: "135"; 
            issue: "39"; pages: "14496-9"
COMMENT     Sequence Label: pMIR-His-Halo-Tev-PTEN
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tagtgaaccg tggatccacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggtgcctcg tggtagccat atggcagaaa tcggtactgg ctttccattc gacccccatt
      121 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
      181 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
      241 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
      301 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
      361 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
      421 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
      481 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
      541 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
      601 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
      661 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
      721 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
      781 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
      841 gcctggc
//
