LOCUS       sequence-92687-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: AAVS1 hPGK-PuroR-pA donor
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..50)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac cgcgcgggca
       61 ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct tcccggccgc
      121 tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc ggtcggcgcg
      181 ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt gcggcttccg
      241 tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg ggcggagcag
      301 gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg ctgcgaacgg
      361 acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa ccacgcccag
      421 agcagccgcg tccctgcgca aacccagggc tgccttggaa aaggcgcaac cccaaaagct
      481 tgccccactg tggggtggag gggacagata aaagtaccca gaaccagagc cacattaacc
      541 ggccctggga atataaggtg gtcccagctc ggggacacag gatccctgga ggcagcaaac
      601 atgctgtcct gaagtggaca taggggcccg ggttggagga agaagactag ctgagctctc
      661 ggacccctgg aagatgccat gacagggggc tggaagagct agcacagact agagaggtaa
      721 ggggggtagg ggagctgccc
//
