LOCUS       sequence-92688-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: AAVS1 hPGK-PuroR-pA donor
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    235..736
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aataaccctc actaaaggga ctagtcctgc aggtttaaac gaattcgccc tttgctttct
       61 ctgaccagca ttctctcccc tgggcctgtg ccgctttctg tctgcagctt gtggcctggg
      121 tcacctctac ggctggccca gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc
      181 actccctctt ccccttgctc tctgctgtgt tgctgcccaa ggatgctctt tccggagcac
      241 ttccttctcg gcgctgcacc acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc
      301 ctctccgggc atctctcctc cctcacccaa ccccatgccg tcttcactcg ctgggttccc
      361 ttttccttct ccttctgggg cctgtgccat ctctcgtttc ttaggatggc cttctccgac
      421 ggatgtctcc cttgcgtccc gcctcccctt cttgtaggcc tgcatcatca ccgtttttct
      481 ggacaacccc aaagtacccc gtctccctgg ctttagccac ctctccatcc tcttgctttc
      541 tttgcctgga caccccgttc tcctgtggat tcgggtcacc tctcactcct ttcatttggg
      601 cagctcccct acccccctta cctctctagt ctgtgctagc tcttccagcc ccctgtcatg
      661 gcatcttcca ggggtccgag agctcagcta gtcttcttcc tccaacccgg gcccctatgt
      721 ccacttcagg acagcatgtt tgctgcctcc agggatcctg tgtccccgag ctgggaccac
      781 cttatattcc cagggccggt taatgtggct ctggttctgg gtacttttat ctgtcccctc
      841 caccccacag t
//
