LOCUS       sequence-9269-ex       10481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC276
FEATURES             Location/Qualifiers
     source          1..10481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    60..184
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(209..214,215..232,233..239)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             293..952
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1294..1599
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1640..1764)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1780..1796)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1781..1797)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(1852..1899)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1975..1995
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2017..2037
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2059..2079
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2101..2121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2143..2820
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             2863..3537
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     protein_bind    complement(3813..3860)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3935..4058
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4099..4404)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(4746..5405)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(join(5459..5465,5466..5483,5484..5489))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5515..5638)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6231..6242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8397..8418
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8581..8685
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8686..8754,8755..9546)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8904..8923)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9717..10305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10206..10225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10459..10476
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatatcaa
       61 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      121 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcat
      181 attggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      241 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      301 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      361 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      421 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      481 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      541 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      601 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      661 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      721 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      781 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      841 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      901 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg
      961 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     1021 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     1081 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     1141 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     1201 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     1261 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     1321 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     1381 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     1441 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     1501 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     1561 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     1621 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     1681 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     1741 ttcagctttc ttgtacaaag tggttgatat caagcttatc gataccgtcg acctcgactc
     1801 tagaccgcgg ttttggcgcg gatccactag ttctagcttc aaaagcgctc tgaagttcct
     1861 atactttcta gagaatagga acttcggaat aggaacttca agatccccgg gtatgtttcg
     1921 aatgatacta acataacata gaacattttc aggagctcag aaaaaatgac tgctccaaag
     1981 aagaagcgta aggtaccggc agaaaaaatg actgctccaa agaagaagcg taaggtaccg
     2041 gcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac cggcagaaaa aatgactgct
     2101 ccaaagaaga agcgtaaggt accggtagac gataaggatc cgatggtggc ctcctccgag
     2161 gacgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
     2221 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2281 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     2341 cagtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgacta caagaagctg
     2401 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2461 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagttccgc
     2521 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2581 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     2641 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     2701 cccgtgcagc tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     2761 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     2821 gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacgtc
     2881 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     2941 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3001 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac
     3061 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc
     3121 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3181 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagtt ccgcggcacc
     3241 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     3301 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     3361 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     3421 cagctgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     3481 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtaggaa
     3541 ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa
     3601 attttaaata ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt
     3661 tttcttgctt tctaaaaaat taattcaatc caaatctaaa catgagcggt tttttttctc
     3721 tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt
     3781 atcttctcgc tttcatttca ttaggacgct ctgaagttcc tatactttct agagaatagg
     3841 aacttcggaa taggaacttc aagatccccg ggtatgtttc gaatgatact aacataacat
     3901 agaacatttt caggagctca ttacgccaag ctatcaactt tgtatagaaa agttgaacga
     3961 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     4021 acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag actggctgtg
     4081 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
     4141 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
     4201 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
     4261 acgggggact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
     4321 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
     4381 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcggca aaagagccgt
     4441 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
     4501 cggcacgcag acgacgggct tcattctgca tggttgtgct taccgaaccg gagatattga
     4561 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
     4621 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata
     4681 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcct
     4741 ctagattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     4801 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     4861 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     4921 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     4981 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     5041 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     5101 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     5161 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     5221 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     5281 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     5341 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     5401 tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa aatccacaca
     5461 ttatacgagc cggaagcata aagtgtaaag cctgggggtg cctaatgcgg ccgccatagt
     5521 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     5581 aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata catagttgat
     5641 aattcactgg gctagccggc catacaagta atccggatga tcgacgccaa cgtcgttgaa
     5701 ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt attctctttg
     5761 tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat gagcggtttt
     5821 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     5881 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     5941 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     6001 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact
     6061 agtctagaat cctatgcttg cactttgagc caatttatcc aagtccttgt aaaaagtatt
     6121 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     6181 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     6241 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     6301 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     6361 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     6421 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     6481 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     6541 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     6601 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     6661 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     6721 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca acaggtttct
     6781 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     6841 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     6901 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     6961 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     7021 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     7081 agcaacaaca atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     7141 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     7201 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     7261 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     7321 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     7381 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     7441 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     7501 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     7561 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     7621 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     7681 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     7741 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     7801 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     7861 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     7921 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     7981 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     8041 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     8101 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     8161 tttcaatttc aactgaaaga ttttttcatt agagaatgtc taggcatgct ctagagggcc
     8221 caaaaggggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8281 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8341 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8401 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8461 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8521 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     8581 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8641 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8701 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8761 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8821 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8881 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8941 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9001 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     9061 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9121 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9181 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9241 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9301 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9361 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9421 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9481 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9541 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9601 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9661 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9721 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9781 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9841 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     9901 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9961 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10021 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10081 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10141 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10201 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10261 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10321 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10381 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    10441 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag c
//
