LOCUS       sequence-92690-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: AAVS1 hPGK-PuroR-pA donor
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    166..695
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 gggcgaattg aatttagcgg ccgcgaattc gcccttagag cagagccagg aacccctgta
       61 gggaaggggc aggagagcca ggggcatgag atggtggacg aggaaggggg acagggaagc
      121 ctgagcgcct ctcctgggct tgccaaggac tcaaacccag aagcccagag cagggcctta
      181 gggaagcggg accctgctct gggcggagga atatgtccca gatagcactg gggactcttt
      241 aaggaaagaa ggatggagaa agagaaaggg agtagaggcg gccacgacct ggtgaacacc
      301 taggacgcac cattctcaca aagggagttt tccacacgga cacccccctc ctcaccacag
      361 ccctgccagg acggggctgg ctactggcct tatctcacag gtaaaactga cgcacggagg
      421 aacaatataa attggggact agaaaggtga agagccaaag ttagaactca ggaccaactt
      481 attctgattt tgtttttcca aactgcttct cctcttggga agtgtaagga agctgcagca
      541 ccaggatcag tgaaacgcac cagacagccg cgtcagagca gctcaggttc tgggagaggg
      601 tagcgcaggg tggccactga gaaccgggca ggtcacgcat cccccccttc cctcccaccc
      661 cctgccaagc tctccctccc aggatcctct ctggctccat cgtaagcaaa ccttagaggt
      721 tctggcaagg agagagatgg ctccaggaaa tgggggtgtg tcaccagata aggaatctgc
      781 ctaacaggag gtgggggtta gacccaatat caggagacta ggaaggagga ggcctaagga
      841 tggggctttt ctgtcaccaa tcctg
//
