LOCUS       sequence-92693-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pcDNA3 vector with added Flag, HA tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     Vectors
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1436 pcDNA3 Flag HA
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(101..127)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(101..127)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(140..163)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(182..201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(246..449)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(275..295)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        450..829
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattccagc acactggcgg ccgttactag tggatccgct ggcgtagtcg ggcacgtcgt
      121 aggggtatcc tccatcgagc ttgtcatcgt cgtccttgta gtccatggta agcttgggtc
      181 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc
      241 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
      301 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
      361 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
      421 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
      481 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
      541 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
      601 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
      661 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
      721 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
      781 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat
      841 ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taaagcagat tcttcatgca
      901 attgtc
//
