LOCUS       sequence-9270-ex       12528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 12528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC245
FEATURES             Location/Qualifiers
     source          1..12528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    91..214
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(255..560)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(902..1561)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1476..1495
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1615..1621,1622..1639,1640..1645))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(1671..1794)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1819..1866)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1942..1962
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1984..2004
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2026..2046
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2068..2088
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2131..2159)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2907..2927
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(3773..3820)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3888..4012
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4441..4746)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(5186..5310)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    5920..6044
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6376..6681)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(7218..7342)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8233..8244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10279..10383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10384..10452,10453..11244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10602..10621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11415..12003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11904..11923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12157..12174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12291..12312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12327..12332,12333..12350,12351..12357)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12365..12381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12370..12392
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12389..12405
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12389..12405
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcctcattac gccaagctat caactttgta tagaaaagtt gaacgagaaa
      121 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      181 aatactgtaa aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata
      241 agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
      301 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
      361 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
      421 gggactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
      481 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
      541 gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcggcaaaag agccgttcat
      601 ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc
      661 acgcagacga cgggcttcat tctgcatggt tgtgcttacc gaaccggaga tattgacatc
      721 atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc
      781 atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc
      841 aaaaatcagc gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatcctctag
      901 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      961 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1021 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1081 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1141 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1201 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1261 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1321 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     1381 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1441 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1501 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1561 ttttagcttc cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat
     1621 acgagccgga agcataaagt gtaaagcctg ggggtgccta atgcggccgc catagtgact
     1681 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     1741 tattgatatt tatatcattt tacgtttctc gttcaacttt attatacata gttgataatt
     1801 cactgggcta gccgctctga agttcctata ctttctagag aataggaact tcggaatagg
     1861 aacttcaaga tccccgggta tgtttcgaat gatactaaca taacatagaa cattttcagg
     1921 agctcagaaa aaatgactgc tccaaagaag aagcgtaagg taccggcaga aaaaatgact
     1981 gctccaaaga agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt
     2041 aaggtaccgg cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggtagaaaaa
     2101 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     2161 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     2221 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg ggtaagttta
     2281 aacatatata tactaactaa ccctgattat ttaaattttc agccaacact tgtcactact
     2341 ttctgttatg gtgttcaatg cttctcgaga tacccagatc atatgaaacg gcatgacttt
     2401 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     2461 gggaactaca agacacgtaa gtttaaacag ttcggtacta actaaccata catatttaaa
     2521 ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     2581 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     2641 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt tgtaagttta
     2701 aacatgattt tactaactaa ctaatctgat ttaaattttc agaacttcaa aattagacac
     2761 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     2821 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     2881 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     2941 acacatggca tggacgaact atacaaaagc ccacaagctt tggaattcgc tagccggcca
     3001 tacaagtaat ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa
     3061 tatttgtttt ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa
     3121 aaaattaatt caatccaaat ctaaacatga gcggtttttt ttctctttcc gtctcccaat
     3181 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca
     3241 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa
     3301 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa
     3361 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat
     3421 taggcgaacg atgagaattg tcctcgcttg ggccctttcg tctcgcgcgt ttcggtgatg
     3481 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     3541 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     3601 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     3661 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata
     3721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgct ctgaagttcc
     3781 tatactttct agagaatagg aacttcggaa taggaacttc aagatccccg ggtatgtttc
     3841 gaatgatact aacataacat agaacatttt caggaggatc cctcgagaca agtttgtaca
     3901 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     3961 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tgaatcaact
     4021 acttagatgg tattagtgac ctgtagtcga ccgacagcct tccaaatgtt cttcgggtga
     4081 tgctgccaac ttagtcgacc gacagccttc caaatgttct tctcaaacgg aatcgtcgta
     4141 tccagcctac tcgctattgt cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa
     4201 gaggtgcgag cctctttttt gtgtgacaaa ataaaaacat ctacctattc atatacgcta
     4261 gtgtcatagt cctgaaaatc atctgcatca agaacaattt cacaactctt atacttttct
     4321 cttacaagtc gttcggcttc atctggattt tcagcctcta tacttactaa acgtgataaa
     4381 gtttctgtaa tttctactgt atcgacctgc agactggctg tgtataaggg agcctgacat
     4441 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
     4501 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
     4561 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
     4621 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
     4681 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
     4741 ctgcatttca ccagtccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg
     4801 cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg
     4861 cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag
     4921 caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag cacacctctt
     4981 tttgacatac ttcgggtagt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     5041 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     5101 aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagtcg actacaggtc
     5161 actaatacca tctaagtagt tgattcatag tgactggata tgttgtgttt tacagtatta
     5221 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     5281 ttctcgttca gctttcttgt acaaagtggt ctcgagaggc ctgttagcca actctagagt
     5341 actcctacag tacccctata gtacagctac agtacctcgc actatccctc catcaattaa
     5401 ttttacagta atcctacagt accccataca gtatcactac aacacctcct actatccctc
     5461 caccaactac ttccacagtt gtcctacagt acccctacag tatcactgca gtacctacta
     5521 ctatctcatc accaactact cctacagtaa tcctaaagta ccatgcacta ttcctccatc
     5581 aactaacctt acagtaatcc tacagtaccg ctacagtacc tcatactatc cctccaccaa
     5641 ttaatcttac agttgtccta cagtacccca tacagtatca ctacagtacc tcctactata
     5701 tcaccaccaa ctacttctac agtactccta cagtacctct atagtgcagc tacagtacct
     5761 catactatcc caccaacaac taatattaca gtaatcctac agtaccccat acagtacctc
     5821 tacactgccc ccgccatgca atatgaacat atcaatagta gaaaaaaatc caattacaga
     5881 ggccttgatc agcggccgcg ggaattcgat tgggtctaga ccactttgta caagaaagct
     5941 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     6001 cagactacat aatactgtaa aacacaacat atccagtcac tatgaatcaa ctacttagat
     6061 ggtattagtg acctgtagtc gactaagttg gcagcatcac ccgacgcact ttgcgccgaa
     6121 taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat
     6181 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca catttcacaa
     6241 ctcttatact tttctcttac aagtcgttcg gcttcatctg gattttcagc ctctatactt
     6301 actaaacgtg ataaagtttc tgtaatttct actgtatcga cctgcagact ggctgtgtat
     6361 aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
     6421 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat
     6481 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
     6541 ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg
     6601 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
     6661 ggtgtaaacc ttaaactgca tttcaccagt ccctgttctc gtcagcaaaa gagccgttca
     6721 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg
     6781 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat
     6841 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
     6901 catagcacac ctctttttga catacttcgg gtatgttctt gatgcagatg attttcagga
     6961 ctatgacact agcgtatatg aataggtaga tgtttttatt ttgtcacaca aaaaagaggc
     7021 tcgcacctct ttttcttatt tctttttatg atttaatacg gcattgagga caatagcgag
     7081 taggctggat acgacgattc cgtttgagaa gaacatttgg aaggctgtcg gtcgactaag
     7141 ttggcagcat cacccgaaga acatttggaa ggctgtcggt cgactacagg tcactaatac
     7201 catctaagta gttgattcat agtgactgga tatgttgtgt tttacagtat tatgtagtct
     7261 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt
     7321 cagctttttt gtacaaactt gtctagaccc aatcactagc cggccataca agtaatccgg
     7381 atgatcgacg ccaacgtcgt tgaattttca aattttaaat actgaatatt tgtttttttt
     7441 cctattattt atttattctc tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat
     7501 ccaaatctaa acatgagcgg ttttttttct ctttccgtct cccaattcgt attccgctcc
     7561 tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt
     7621 ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac
     7681 acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa
     7741 atatatagag gaggaaggaa aactagtaaa aaataagcaa agaaattagg cgaacgatga
     7801 gaattgtcct cgcttgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7861 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7921 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     7981 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     8041 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     8101 ggttaatgtc atgataataa tggtttctta gacaacggat aaaatgttta ttataatatc
     8161 aaaaacaatt tgtcagttga ccactttttg atatatttga cagaaaccgg gatgaattgg
     8221 ctcaaagtta gggcgccctt ctattacagg gtttctgata acaaaccggt tattaactcc
     8281 caacaaggga tgatttcaat tcatcatgct caaattgacc caaattaagt tacatgacaa
     8341 attcatcgcc cttttcaact cttcttggct catcatctgt tattctgttc ctattctgtc
     8401 tgcaccctat acccctttgc atactctctc gtcattcctt tcttggatag tgtcttcttc
     8461 tccccagctc tgctacttct atgacttgcg cccgctgctt ttccgctcgt tcctctcctc
     8521 tctgacgtct tcttctgctt cttttctcct tcctttttcc cttctcaatc ttcctctttt
     8581 tcccatttct gtcaatcatt cgaagaagaa gaagaagaag accctcattc atttcatttt
     8641 tttttctgtc ggtgtgtgct gctcggttaa gagtgagctc tctattccac gtcttcttct
     8701 ttttcttctt cgattcgaat caatcactcc accaaaacgc attcgttttt gggattcacc
     8761 ccgcggttcg caacaggttt ctttttcaaa ttattagcgt tataaataga aaaatggtgg
     8821 agtttcaata aaaatgataa tttacaaagt gtattttgat tacatgtact caaaaaggtt
     8881 gaaatttttc ataccagttt tccggaaatc catctgatat cattatcgta tttttctttt
     8941 taaaaatgtt ttcaaaaaaa aacaaaatat agctggttat ttggcaccct ctaattacca
     9001 ttttcctgtc acaccacact cttttccttc ttccacttct ttccgttttc agccgcttcc
     9061 aaccaaaccg atatgaaagc cgagcaacaa caatcgattc cacccggctc ggcgaccttc
     9121 ccgtcgcagg tgagactcag aaaactagag aaaccgctca actaactctt gatatccgat
     9181 ttcattcttt tctcttttct ttttgttgaa cttttccata tttccagatg ccacggccac
     9241 caccaagcac cgaacaagga atcacaacgg aatcggagct tgcgaagaaa gctcaaatca
     9301 ctccgaacga cgttttagca cttccgggaa tcactcaagg tatcttgctc cctttggatt
     9361 tcagtttaaa acataaattt aggattctta tgctccccat ctgcgaacat ctataacatc
     9421 gagttcacca agttccaaat ccgtgatctg gacactgagc aagtgctttt cgagatcgcc
     9481 aaaccggaga acgatcagga gaatgatgag tcgccacagg agtcggcaag atacgtgcgt
     9541 tatagatttg ctccaaactt tttgaaactc aaaacggtcg gagcaactgt ggaattcaaa
     9601 gtaggagaca tcccaatcca tcatttccga atgatcgaac gtcacttctt caaagatcgc
     9661 cttctgaagt gttttgactt tgaattcgga ttctgtattc cgaattcacg aaacaactgt
     9721 gaacatatct atgagttccc tcaactctct caacaactca gtgagtcatt attctaaaaa
     9781 gtacaattca aaagactaat ctctttttca gtggacgaca tgatcaacaa tccaaacgag
     9841 actcgttctg acagcttcta tttcgtcgat aacaaactcg tcatgcacaa caaagccgac
     9901 tactcatatg atgcataaat atttaataca aaaatgttct ggataattat tctgtcgaat
     9961 agaaaaaaaa ctccaaatgt gattaaattc caataattcc tgtctagttt gttccttccc
    10021 cttccccttc tcatgttcaa tgcattccta agcttttcag ttcccccctt gtttctatat
    10081 tttttcgctg tcctgtcaca ctcgctaaaa acactaatca cacggaaatc tgttttcaat
    10141 aaaaactcca actttaactc attttcaatt tcaactgaaa gattttttca ttagagaatg
    10201 tctaggcatg ctctagaggg cccaaaaggc cttttttttt tggcgccgac gtcaggtggc
    10261 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    10501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    10561 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    10621 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    10681 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    10741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    10801 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    10861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    10921 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10981 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    11041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11221 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11281 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11341 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    11401 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    11461 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    11521 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
    11581 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    11641 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11701 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11761 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
    11821 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11881 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11941 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    12001 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    12061 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    12121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    12181 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    12241 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    12301 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    12361 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    12421 ctgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa
    12481 aaatggctga aatcactcac aacgatggat acgctaacaa cttggaaa
//
