LOCUS       sequence-92704-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD 
            double mutant S205D, K224A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: pSG5-Myr-FLAG-p110-beta-DM
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..62
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accccagcca gcgccggcgc aggatccgag gttaccttga ttacaaggat gacgacgata
       61 agcctcctgc tatggcagac aaccttgaca tctgggcagt ggactcacag attgcatccg
      121 atggcgccat atccgtcgat ttccttctgc ccaccgggat ttatatccag ttggaagtac
      181 ctcgggaagc taccatttct tatattaaac agatgttatg gaagcaagtt cacaactacc
      241 cgatgtttaa cctcctcatg gacattgact cgtatatgtt tgcatgtgtg aatcaaactg
      301 ctgtatatga ggaactggaa gacgaaacac gaagactttg tgatgtcaga ccttttcttc
      361 cagttctcaa actagtgact agaagctgtg accccgcaga aaaattggac tcaaaaattg
      421 gggttcttat aggaaaaggt cttcatgagt ttgatgcctt gaaggatccc gaagtgaatg
      481 aatttagaag aaaaatgcgc aaattcagtg aggccaagat tcagtctctg gtagggttgt
      541 cttggatcga ctggctaaag cacacgtatc cgcctgagca cgagccgtcc gtcctggaga
      601 acttggaaga taaactttat ggaggaaagc tggttgtggc tgtgcacttt gaaaatagcc
      661 aggatgtatt tgattttcaa gtgtctccca atttgaatcc tataaaaata aatgaattgg
      721 caatccaggc acgcctcact attcgtggaa aggaagatga agctagcccc tgtgactatg
      781 tgttacaggt cagtgggaga gtggagtatg tgtttggcga tcatccacta attcagttcc
      841 agtacatccg gaattgtgtg atgaatagaa ccctgcccca cttcatcctt gtggaatgtt
      901 gtaagatc
//
