LOCUS       sequence-9271-ex       10246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC320
FEATURES             Location/Qualifiers
     source          1..10246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    91..214
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(255..560)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(902..1561)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1476..1495
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1615..1621,1622..1639,1640..1645))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(1671..1794)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1819..1866)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1942..1962
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1984..2004
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2026..2046
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2068..2088
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2101..2811
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2246..2264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2514..2533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3602..3649)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3717..3841
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4270..4575)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(5015..5139)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    5749..5873
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6205..6510)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(7047..7171)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        7991..8095
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8096..8164,8165..8956)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8314..8333)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9127..9715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9616..9635
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9869..9886
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10003..10024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10039..10044,10045..10062,10063..10069)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10077..10093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10082..10104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10101..10117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10101..10117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcctcattac gccaagctat caactttgta tagaaaagtt gaacgagaaa
      121 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      181 aatactgtaa aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata
      241 agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
      301 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
      361 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
      421 gggactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
      481 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
      541 gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcggcaaaag agccgttcat
      601 ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc
      661 acgcagacga cgggcttcat tctgcatggt tgtgcttacc gaaccggaga tattgacatc
      721 atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc
      781 atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc
      841 aaaaatcagc gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatcctctag
      901 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      961 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1021 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1081 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1141 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1201 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1261 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1321 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     1381 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1441 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1501 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1561 ttttagcttc cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat
     1621 acgagccgga agcataaagt gtaaagcctg ggggtgccta atgcggccgc catagtgact
     1681 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     1741 tattgatatt tatatcattt tacgtttctc gttcaacttt attatacata gttgataatt
     1801 cactgggcta gccgctctga agttcctata ctttctagag aataggaact tcggaatagg
     1861 aacttcaaga tccccgggta tgtttcgaat gatactaaca taacatagaa cattttcagg
     1921 agctcagaaa aaatgactgc tccaaagaag aagcgtaagg taccggcaga aaaaatgact
     1981 gctccaaaga agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt
     2041 aaggtaccgg cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggtagaaaaa
     2101 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     2161 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     2221 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     2281 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     2341 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     2401 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     2461 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     2521 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     2581 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     2641 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     2701 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     2761 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agaattcgct
     2821 agccggccat acaagtaatc cggatgatcg acgccaacgt cgttgaattt tcaaatttta
     2881 aatactgaat atttgttttt tttcctatta tttatttatt ctctttgtgt tttttttctt
     2941 gctttctaaa aaattaattc aatccaaatc taaacatgag cggttttttt tctctttccg
     3001 tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc
     3061 tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga
     3121 tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg
     3181 aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag
     3241 caaagaaatt aggcgaacga tgagaattgt cctcgcttgg gccctttcgt ctcgcgcgtt
     3301 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     3361 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     3421 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     3481 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc
     3541 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgctc
     3601 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca agatccccgg
     3661 gtatgtttcg aatgatacta acataacata gaacattttc aggaggatcc ctcgagacaa
     3721 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     3781 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     3841 gaatcaacta cttagatggt attagtgacc tgtagtcgac cgacagcctt ccaaatgttc
     3901 ttcgggtgat gctgccaact tagtcgaccg acagccttcc aaatgttctt ctcaaacgga
     3961 atcgtcgtat ccagcctact cgctattgtc ctcaatgccg tattaaatca taaaaagaaa
     4021 taagaaaaag aggtgcgagc ctcttttttg tgtgacaaaa taaaaacatc tacctattca
     4081 tatacgctag tgtcatagtc ctgaaaatca tctgcatcaa gaacaatttc acaactctta
     4141 tacttttctc ttacaagtcg ttcggcttca tctggatttt cagcctctat acttactaaa
     4201 cgtgataaag tttctgtaat ttctactgta tcgacctgca gactggctgt gtataaggga
     4261 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
     4321 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt
     4381 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
     4441 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc
     4501 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
     4561 aaccttaaac tgcatttcac cagtccctgt tctcgtcagc aaaagagccg ttcatttcaa
     4621 taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca
     4681 gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata
     4741 tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc
     4801 acacctcttt ttgacatact tcgggtagtg ccgatcaacg tctcattttc gccaaaagtt
     4861 ggcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
     4921 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttagtcga
     4981 ctacaggtca ctaataccat ctaagtagtt gattcatagt gactggatat gttgtgtttt
     5041 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     5101 attttacgtt tctcgttcag ctttcttgta caaagtggtc tcgagaggcc tgttagccaa
     5161 ctctagagta ctcctacagt acccctatag tacagctaca gtacctcgca ctatccctcc
     5221 atcaattaat tttacagtaa tcctacagta ccccatacag tatcactaca acacctccta
     5281 ctatccctcc accaactact tccacagttg tcctacagta cccctacagt atcactgcag
     5341 tacctactac tatctcatca ccaactactc ctacagtaat cctaaagtac catgcactat
     5401 tcctccatca actaacctta cagtaatcct acagtaccgc tacagtacct catactatcc
     5461 ctccaccaat taatcttaca gttgtcctac agtaccccat acagtatcac tacagtacct
     5521 cctactatat caccaccaac tacttctaca gtactcctac agtacctcta tagtgcagct
     5581 acagtacctc atactatccc accaacaact aatattacag taatcctaca gtaccccata
     5641 cagtacctct acactgcccc cgccatgcaa tatgaacata tcaatagtag aaaaaaatcc
     5701 aattacagag gccttgatca gcggccgcgg gaattcgatt gggtctagac cactttgtac
     5761 aagaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     5821 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atgaatcaac
     5881 tacttagatg gtattagtga cctgtagtcg actaagttgg cagcatcacc cgacgcactt
     5941 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
     6001 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
     6061 atttcacaac tcttatactt ttctcttaca agtcgttcgg cttcatctgg attttcagcc
     6121 tctatactta ctaaacgtga taaagtttct gtaatttcta ctgtatcgac ctgcagactg
     6181 gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt
     6241 gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac
     6301 actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta
     6361 aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg
     6421 tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc
     6481 tcttttatag gtgtaaacct taaactgcat ttcaccagtc cctgttctcg tcagcaaaag
     6541 agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc
     6601 agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga
     6661 tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat
     6721 acgctgcttc atagcacacc tctttttgac atacttcggg tatgttcttg atgcagatga
     6781 ttttcaggac tatgacacta gcgtatatga ataggtagat gtttttattt tgtcacacaa
     6841 aaaagaggct cgcacctctt tttcttattt ctttttatga tttaatacgg cattgaggac
     6901 aatagcgagt aggctggata cgacgattcc gtttgagaag aacatttgga aggctgtcgg
     6961 tcgactaagt tggcagcatc acccgaagaa catttggaag gctgtcggtc gactacaggt
     7021 cactaatacc atctaagtag ttgattcata gtgactggat atgttgtgtt ttacagtatt
     7081 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     7141 tttctcgttc agcttttttg tacaaacttg tctagaccca atcactagcc ggccatacaa
     7201 gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa attttaaata ctgaatattt
     7261 gttttttttc ctattattta tttattctct ttgtgttttt tttcttgctt tctaaaaaat
     7321 taattcaatc caaatctaaa catgagcggt tttttttctc tttccgtctc ccaattcgta
     7381 ttccgctcct ctcatctgaa cacaatgtgc aagtttattt atcttctcgc tttcatttca
     7441 ttaggacgtg gggggaattg gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa
     7501 taaggacaca caatatgcaa caacattcaa ttcagaaata tggaggaagg tttaaaagaa
     7561 aacataaaaa tatatagagg aggaaggaaa actagtaaaa aataagcaaa gaaattaggc
     7621 gaacgatgag aattgtcctc gcttgggccc tttcgtctcg cgcgtttcgg tgatgacggt
     7681 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     7741 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     7801 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     7861 cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct
     7921 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7981 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8041 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8101 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8161 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8221 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8281 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8341 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8401 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8461 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8521 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8581 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8641 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8701 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8761 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8821 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8881 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8941 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9001 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9061 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9121 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9181 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9241 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     9301 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9361 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9421 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9481 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     9541 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9601 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9661 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9721 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9781 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9841 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9901 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9961 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    10021 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    10081 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt
    10141 ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
    10201 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaat
//
