LOCUS       sequence-92733-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector (pT7) for untagged expression as well as fusions 
            to His6-SUMO or His6 tags. Designed for single or multiple fragment 
            cloning by yeast homologous recombination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Holmberg MA, Gowda NK, Andreasson C
  TITLE     A versatile bacterial expression vector designed for single-step 
            cloning of multiple DNA fragments using homologous recombination.
  JOURNAL   Protein Expr Purif. 2014 Jun;98:38-45. doi: 
            10.1016/j.pep.2014.03.002. Epub 2014 Mar 12.
  PUBMED    24631626
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2014-06"; volume: "98:38-45. doi"; pages: " 
            10.1016/j.pep.2014.03.002. Epub 2014 Mar 12"
COMMENT     Sequence Label: pSUMO-YHRC
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(88..378)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             complement(382..399)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             413..435
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    450..474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(474..493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(475..493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(577..596)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        802..879
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc
       61 gacggagctc gaattcggat ccggtctccc accaatctgt tctctgtgag cctcaataat
      121 atcgttatcc tccatgtcca aatcttcagg ggtctgatca gcttgaattc taataccgtc
      181 gtacaagaat cttaaggagt ccatttcctt accctgtctt ttagcgaacg cttccatcag
      241 ccttcttaaa ggagtggtct ttttgatctt gaagaagatc tctgaagatc catcggacac
      301 ctttaaattg atgtgagtct caggcttgac ttctggcttg acctctggct tagcttcttg
      361 attgacttct gagtccgaac catggtgatg atggtgatga cccatatgta tatctccttc
      421 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      481 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg
      541 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
      601 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
      661 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
      721 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
      781 agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca
      841 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac
      901 gatgt
//
