LOCUS       sequence-92734-e        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR 
            experiments. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello
            CC
  TITLE     A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2014 May 30. pii: genetics.114.166389.
  PUBMED    24879462
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 May 30. pii: genetics.114.166389."
COMMENT     Sequence Label: pCCM935
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    101..337
                     /label=3' CeU6
                     /note="region downstream of the C. elegans K09B11.12 U6 
                     snRNA gene"
     misc_RNA        complement(345..420)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(441..940)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     promoter        complement(1027..1045)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1028..1045)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1063..1079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1063..1079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tgggccctct agatgcatgc tcgagcggcc
       61 gccagtgtga tggatatctg cagaattcgc ccttaagctt cacagccgac tatgtttggc
      121 gtcgagattt tgtaattttc tttggaatat tgtcaaaaaa aatcaatttt ttttcgagtt
      181 tcctaataaa attttttgga ccaatttaat caactctggc aactttaaaa tcgtccctcc
      241 gggaatttac ctttaaccaa gattagttga aacattgaac tcaaaaattt agagtatttc
      301 attttagttt caaaaaggga tatctagaat attattgaaa aaaagcaccg actcggtgcc
      361 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac
      421 gtgtattcgg caacgggttc aaacatttag atttgcaatt caattatata gggaccattt
      481 atcatgcagc tgacatattg agagtcctgt aagacactat accaaatggg gatgtagatc
      541 aataatttgt gggggaggca tgtgtgattc gtactcgttc ggatagagct ttggtgaatg
      601 tccaggcttc tgaaacgtag gtgagcgctg gaaggacaat tgagtcgaac agattcgcac
      661 gaatcttctt gtcggtgatg gagtcggtgg cattcttgat tccattgaat gcagcccagg
      721 ctgctcgacg tcttcggtgg atttccggca tcaagttgtt ttgggcgttg atttgacgac
      781 cgaggtagat gtactcgtcg acgtcgtcga gctgggtggt gggggaaggg ctaccgaagt
      841 agactttact ggggtcagcg aatcggtttc gcaagacttt cgtcttccca gtattgatct
      901 cgagacctac ttcagagcat ttttgtacga gttcttggag gaattcaaag ggcgaattcc
      961 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga tgcatagctt
     1021 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctg
//
