LOCUS       sequence-92735-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR 
            experiments. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello
            CC
  TITLE     A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2014 May 30. pii: genetics.114.166389.
  PUBMED    24879462
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 May 30. pii: genetics.114.166389."
COMMENT     Sequence Label: pCCM935
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..39
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     21..38
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        126..625
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     misc_RNA        646..721
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct atgcatcaag
       61 cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat tcgccctttg
      121 aattcctcca agaactcgta caaaaatgct ctgaagtagg tctcgagatc aatactggga
      181 agacgaaagt cttgcgaaac cgattcgctg accccagtaa agtctacttc ggtagccctt
      241 cccccaccac ccagctcgac gacgtcgacg agtacatcta cctcggtcgt caaatcaacg
      301 cccaaaacaa cttgatgccg gaaatccacc gaagacgtcg agcagcctgg gctgcattca
      361 atggaatcaa gaatgccacc gactccatca ccgacaagaa gattcgtgcg aatctgttcg
      421 actcaattgt ccttccagcg ctcacctacg tttcagaagc ctggacattc accaaagctc
      481 tatccgaacg agtacgaatc acacatgcct cccccacaaa ttattgatct acatccccat
      541 ttggtatagt gtcttacagg actctcaata tgtcagctgc atgataaatg gtccctatat
      601 aattgaattg caaatctaaa tgtttgaacc cgttgccgaa tacacgtttt agagctagaa
      661 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      721 ctttttttca ataatattct agatatccct ttttgaaact aaaatgaaat actctaaatt
      781 tttgagttca atgtttcaac taatcttggt taaaggtaaa ttcccggagg gacgatttta
      841 aagttgccag agttgattaa attggtccaa aaaattttat taggaaactc gaaaaaaaat
      901 tgattttttt tgacaatatt ccaaagaaaa ttacaaa
//
