LOCUS       sequence-92744-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN S113L mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN S113L
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             593..622
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             641..664
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(684..700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(684..700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcagagctc gttagtgacc gtcagaattt tgtaatacga ctcactatag ggcggccggg
       61 aattcgtcga ctggatccgg taccgaggag atctgccgcc gcgatcgcca tggtcctgag
      121 tggggcgctg tgcttccgaa tgaaggactc ggcattgaag gtgctttatc tgcataataa
      181 ccagcttcta gctggagggc tgcatgcagg gaaggtcatt aaaggtgaag agatcagcgt
      241 ggtccccaat cggtggctgg atgccagcct gtcccccgtc atcctgggtg tccagggtgg
      301 aagccagtgc ctgtcatgtg gggtggggca ggagccgact ctaacactag agccagtgaa
      361 catcatggag ctctatcttg gtgccaagga atccaagagc ttcaccttct accggcggga
      421 catggggctc acctccagct tcgagttggc tgcctacccg ggctggttcc tgtgcacggt
      481 gcctgaagcc gatcagcctg tcagactcac ccagcttccc gagaatggtg gctggaatgc
      541 ccccatcaca gacttctact tccagcagtg tgacacgcgt acgcggccgc tcgagcagaa
      601 actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg atgacgacga
      661 taaggtttaa acggccggcc gcggtcatag ctgtttcctg aacagatccc gggtggcatc
      721 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
      781 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
      841 ttatggggtg g
//
