LOCUS       sequence-92745-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN S113L mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN S113L
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..58
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     42..58
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(78..101)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(120..149)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(690..709)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(691..709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(735..940)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(764..784)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cactggggaa ggggtcacag ggatgccacc cgggatctgt tcaggaaaca gctatgaccg
       61 cggccggccg tttaaacctt atcgtcgtca tccttgtaat ccaggatatc atttgctgcc
      121 agatcctctt ctgagatgag tttctgctcg agcggccgcg tacgcgtgtc acactgctgg
      181 aagtagaagt ctgtgatggg ggcattccag ccaccattct cgggaagctg ggtgagtctg
      241 acaggctgat cggcttcagg caccgtgcac aggaaccagc ccgggtaggc agccaactcg
      301 aagctggagg tgagccccat gtcccgccgg tagaaggtga agctcttgga ttccttggca
      361 ccaagataga gctccatgat gttcactggc tctagtgtta gagtcggctc ctgccccacc
      421 ccacatgaca ggcactggct tccaccctgg acacccagga tgacggggga caggctggca
      481 tccagccacc gattggggac cacgctgatc tcttcacctt taatgacctt ccctgcatgc
      541 agccctccag ctagaagctg gttattatgc agataaagca ccttcaatgc cgagtccttc
      601 attcggaagc acagcgcccc actcaggacc atggcgatcg cggcggcaga tctcctcggt
      661 accggatcca gtcgacgaat tcccggccgc cctatagtga gtcgtattac aaaattctga
      721 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      781 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
      841 aacaaactcc cattgacgtc aatggggtgg agacttggaa aatccccgtg agtcaaaccg
      901 ctatccacgc ccattggtgt actgccaaaa accgcatcac catggtaata gcgatgacta
      961 atacg
//
