LOCUS       sequence-92747-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN R48W mutant .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN R48W
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             586..615
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             634..657
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(677..693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(677..693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcgttagtga accgtcagaa ttttgtaata cgactcacta tagggcggcc gggaattcgt
       61 cgactggatc cggtaccgag gagatctgcc gccgcgatcg ccatggtcct gagtggggcg
      121 ctgtgcttcc gaatgaagga ctcggcattg aaggtgcttt atctgcataa taaccagctt
      181 ctagctggag ggctgcatgc agggaaggtc attaaaggtg aagagatcag cgtggtcccc
      241 aattggtggc tggatgccag cctgtccccc gtcatcctgg gtgtccaggg tggaagccag
      301 tgcctgtcat gtggggtggg gcaggagccg actctaacac tagagccagt gaacatcatg
      361 gagctctatc ttggtgccaa ggaatccaag agcttcacct tctaccggcg ggacatgggg
      421 ctcacctcca gcttcgagtc ggctgcctac ccgggctggt tcctgtgcac ggtgcctgaa
      481 gccgatcagc ctgtcagact cacccagctt cccgagaatg gtggctggaa tgcccccatc
      541 acagacttct acttccagca gtgtgacacg cgtacgcggc cgctcgagca gaaactcatc
      601 tcagaagagg atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt
      661 taaacggccg gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga
      721 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
      781 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
      841 gtg
//
