LOCUS       sequence-92754-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN P76L mutant  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN P76L
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..70
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(90..113)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(132..161)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(702..721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(703..721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(747..950)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(776..796)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agggccagga aagcactggg gaggggtcac agggatgcca cccgggatct gttcaggaaa
       61 cagctatgac cgcggccggc cgtttaaacc ttatcgtcgt catccttgta atccaggata
      121 tcatttgctg ccagatcctc ttctgagatg agtttctgct cgagcggccg cgtacgcgtg
      181 tcacactgct ggaagtagaa gtctgtgatg ggggcattcc agccaccatt ctcgggaagc
      241 tgggtgagtc tgacaggctg atcggcttca ggcaccgtgc acaggaacca gcccgggtag
      301 gcagccgact cgaagctgga ggtgagcccc atgtcccgcc ggtagaaggt gaagctcttg
      361 gattccttgg caccaagata gagctccatg atgttcactg gctctagtgt tagagtcagc
      421 tcctgcccca ccccacatga caggcactgg cttccaccct ggacacccag gatgacgggg
      481 gacaggctgg catccagcca ccgattgggg accacgctga tctcttcacc tttaatgacc
      541 ttccctgcat gcagccctcc agctagaagc tggttattat gcagataaag caccttcaat
      601 gccgagtcct tcattcggaa gcacagcgcc ccactcagga ccatggcgat cgcggcggca
      661 gatctcctcg gtaccggatc cagtcgacga attcccggcc gccctatagt gagtcgtatt
      721 acaaaattct gacggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc
      781 ctaccgccca tttgcgtcaa cggggcgggg ttattacgac attttggaaa gtcccgttga
      841 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
      901 gagtcaaacc gctatccacg cccattggtg tactgccaaa accgcatcac catggtaata
      961 gcgatgacta atacgtaaat gtactgccaa gtagga
//
