LOCUS       sequence-92755-e       14288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in 
            neurons in zebrafish larva. The plasmid is in Tol2 transposon 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14288)
  AUTHORS   Freeman J, Vladimirov N, Kawashima T, Mu Y, Sofroniew NJ, Bennett 
            DV, Rosen J, Yang CT, Looger LL, Ahrens MB
  TITLE     Mapping brain activity at scale with cluster computing.
  JOURNAL   Nat Methods. 2014 Jul 27. doi: 10.1038/nmeth.3041.
  PUBMED    25068736
REFERENCE   2  (bases 1 to 14288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jul 27. doi: 10.1038/nmeth.3041."
COMMENT     Sequence Label: Tol2-elavl3-H2B-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..14288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             9455..9832
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      9845..9854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9851..9859,9860..9877,9878..9880,9881..9913,9914..9916,
                     9917..9940,9941..9967,9968..10024,10025..10030,10031..10300,
                     10301..10318,10319..10753,10754..10759,10760..11203)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      9947..9956
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     10237..10258
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(10364..10385)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10625..10644)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    11245..11379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11269..11288)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11323..11342
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(11818..12005,12006..12017)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(12051..12067)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12051..12067)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12104..12124)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12104..12122)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12143..12159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12143..12159)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12156..12178)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12167..12183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12191..12197,12198..12215,12216..12221))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12236..12257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12374..12391)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12545..13133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12625..12644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13304..14095,14096..14164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13927..13946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14165..14269)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttcccg
      721 ggtgtcgacg aattctcgag ctattcctaa agaccttggg tgaccaaaat cttattttaa
      781 taaataaaac tgtttattaa aacttttttg tttcaaagaa ccatatgtat agtgaaattt
      841 ataaaaatat caatttttaa aaagctggtg tactcattta tgttatgaac tctaaaacca
      901 tatactgact gcaagtgatg atgtatagag tgatgtttac gagtaaacat atttagttgt
      961 atacatccta ctgagcacat tttgatgtat gaaataacat tacaagcttt atccaaatta
     1021 agccatttta aaacactgcc aattgaaaat acaaatcctg gaaaaaatcg tctttagcgc
     1081 agtcatttga gccatcctaa tccgttacct cagaccataa taagaaggga taacactagc
     1141 tgtagcaatg gaacacatct gtttcacaca atcatatctc ctgcgccggt gctaagcaga
     1201 ttcagcgtga tcataacatg ctttccactc ataaatgtaa atttacaatt tgcacatgta
     1261 aaacagacac ttttgagata ttggataaaa aaacaagagt atattgctta gtttcatcca
     1321 ccagtcatcc ccacagcgtt tggaaggcca taaaaagtgt ctaaaatcaa tgatcattga
     1381 aagagcacaa gagagactct tacgctgtaa tgccactggg gacaaaagtg acagtctctt
     1441 aatgggctct tctggagggg ctcctgaaca ttaaaaatta tcagcgaaat taccgaaaga
     1501 gcttcaagca actggcatgc ttgatcctct gcgtcggggc ggtgaatagg tgcttcagat
     1561 gccctcttac ccacgggctg gattcagctg ccccgctacc agcggagacc ccctaatgag
     1621 cctctgcaat taagtttatt catgttaagt gtgaacgggg tgcgtgcgga actgtgggca
     1681 gctaacagac ctgggttctt tgtgccacaa gtgctgcctt tattcggctc acaaagcaga
     1741 aaacaacacc cgcacctata atggcgccct cggctgggtc taagaaacgt ggcgagttga
     1801 cagagcagag tgggcggggt taagacagac tgacagcggg acccatctcc atcctcttat
     1861 taacgcttaa cgagtgcctt cctcatgcaa tattcatcgc cactaatatc atccaagctc
     1921 tgagctgagc tggccactta tgtaaggcaa ttatgtaaaa tatcagacag ggcccacact
     1981 cagaatctga ctggggtaga gacgcgggac gagaaccgag agcaagaact gaaagtgaaa
     2041 gtgaccacta aagggaggag aggacagagg ggcaggatgt gtcaagatta ccagagaaca
     2101 cttggccaga aatgcgcaac cattggagct ctccggatta cccaaaggtt aacgagtttg
     2161 aacgcctctg cccactcgcc catctctgat ggtttcccaa gaactcctca agcaaaatat
     2221 atataattgt gtgtattatg cacagacacg agaaaatgct gtttttctga tctgcattac
     2281 agcacatttg cccgccaacg acaataccac ccactcggta cctcgctgac tcctgatgcc
     2341 tgatacctgc gcggtgactg tctacaatct gcataatcaa gagaagttgt gttgaagacg
     2401 agcgccacac aaccgtttcc acaaggtcac ccaaggccgg tgcagatgta ggtgaggtct
     2461 ccataaacag actgaaataa acacatcctc cgctgggaac aacaaccccc tcacgcctca
     2521 tgcatttcca taagcctaca tgcatctctt ccaacttatg gagactcgca cctaccaaca
     2581 tccgcacaac aaagatatac agagcgcgct ccctcaggtc aaggccctgt gggggtctgt
     2641 gcagaaatag gtcatttgtc acacatcaag tcctggggca ggagatgcat tatagatgag
     2701 accaaacagc ctgtctcggt gagctctacc cactccctga gactagaaat gggggaaggg
     2761 agcttgagat aacaaccgct gcaatcactg tgtcgatgtt taatatcagc accaaccggg
     2821 aacaataagg agatgggtgc attcatgttc acatcttacc agtcaagtat catcgaaccg
     2881 gcttgataac cacacctcgt gtaatagctg agcagatagt tgtcatttta aagcgttggc
     2941 ctttgtcgat tatgtaatgc gcacattcaa cacatggtaa tatagaaacg gttatgtcga
     3001 ggttgttttg tccagagatg accttcacac agttacagcc gctctgcatc cacacaaatg
     3061 gaggacttaa tcgtggactg cattcttaga aatgatctac aaagacaaat aatgtgaaat
     3121 caagaaagga caaaatttaa gtaaggggat gagggagaga gagaacgagg ggcaaggaga
     3181 aagcatggct cctgtctttt tctgcaccca tctgttcgga gtgcaggtgg agctctattc
     3241 actcagctct gcatgtgtgt ttgggggggg caggaagaaa gggagggcaa aaggaagagt
     3301 ggagagatgg tgggggctgg agggatgggg ggttctcggt gatctctcct gaaggggata
     3361 atgggagagc agcgctttgc aatggctgcc atgtagtacc ctccctgcac aattagccaa
     3421 tcagcagcaa gctctgccag ccagaaggac acataaaaga agaacattgc agcagaggca
     3481 cagaaggagc ctgcgaggag ctgggaaata cacacacaac agcagaacca caacaccctc
     3541 ccctggacac accctactgg ggatcactgc ttttcttttt ttctgaacca tcgcccacgc
     3601 cacacggaga gaaatctctc tctcatcatc atcctgaaga aaaccccctt atcctcattt
     3661 tcacactgct gaggaaaacc tacaatcgca cgggctgaga tttcctggcg aagactgtcc
     3721 tttttccttt ttcttttttt ttcttttcct ttggaaactg acatttgcat ttctccattc
     3781 caagccacgg cgtaataata tctgcaatcc agcctgaaga cctgcaaatc gaaggaccta
     3841 gatcactatc atctttgtac gtcaagaatg gttactgtac gtataacctt tctttctttt
     3901 gctctgacca atatgaaaca ctaaaatcga ttcgagcagc ctctcagcat caattacagt
     3961 gcgtgaaaaa cattcaaata gaggcagcaa atattacatg tgaaaataca ggctggctaa
     4021 atccaagcta atttagaaat gtggtcaaaa cgcatactgg cacgtctaat cgcggattca
     4081 gtaaacaaga ttaacgatta gcccagtgta taagtcatat gatacaggca tgcgcgagag
     4141 catcgctaca cccgagctgg cttcattttc ggaggaaaat caaaacattg ctttctcctg
     4201 ccgtgcgaac cattcgtcat aaaccgtaat acgcaacata catttattac tacatccgtt
     4261 aattagcgat aattagccgt tattaacaaa gagcgctgag gaattcctct caaatagcgg
     4321 aggtgcggcg gaggcagagg ggcgtaaaag ggcacatgcg tgcctggctc aaaaaaggat
     4381 gctccagact gaaactcaga gacaaaacac gatgcctcgg aggctgagag cccatcgaga
     4441 ggaaaggcaa agaaaagggt cgagtcactc agagggggag gggagatatt gtgcatccat
     4501 tggtttgaat tgaagcaggc agaataaaaa tccttgcgat tatgcttcgt gagagatcgc
     4561 gagagaggaa aaggctaaat gccacgtatg caaatggata aatgtcatct atttcttcgc
     4621 gagtagacat tttgtggaca acgagagtgt aaataagcga tatgagggga atacaaaggc
     4681 cttgagggaa agcgttttgg gcgtgtcttg tcgaaaagaa gacagcgatg gcacgcgctc
     4741 agaaccacca tcttcacgta gataatccgc gtgaaaacaa taacaaaact gctcatttta
     4801 acacgagcag gtgtttgaaa ccacccaaaa aagattgtct aaaatattaa gattaattaa
     4861 tgacgtttaa atgtccaggc ctgatataaa tgaagcgtgt agtaagagta atcgcctttg
     4921 tttcttttca gagatacgtt ttcagagatt cagggaatta tatcttcaat aaaataatgt
     4981 ttgatttcat tattttaaag ctttttaaga agtaaaaata cacattattc atttacattt
     5041 tattttattt aaaggcctat ggtcttgaaa ataataagtg gaatatatag gctatatatt
     5101 cgtctaagga cagccggtgt tattttatag tgacctcaga caaaagccga gacaaaacga
     5161 ggcgcttgcc tttcatttta atccttaata gacggtgtga tgaatgaatg aatgaatgaa
     5221 taaatgaatg aatgacgggc ccttcggcat gttgttccgc agtcctccgt gagcacgagt
     5281 ctctaccgca gtgccaagca cataaaaccg ttgagtcaga taaccttctc caggcgcgta
     5341 tacacaattc aaggacaagc acatcacaac tatacacaac acaacacaac cgcctaaacg
     5401 cacgagctca tcaatcgcaa tttaaaggca tgtcagtcaa agaaaaaggc ggcgttattt
     5461 aaaaccaatg ttcaacaata tttgtatgct gactagcccg ggcgcgcacg ctataagatc
     5521 acaaggcctt gttttttccc tttattcaaa atatcattaa ttattacagg tcataaaaca
     5581 aaacaatgca aattacaatg atttaaagac tattgattta acaataatca gttaattata
     5641 gaataatatt ataatatttt acatacaaat gaaagatgac attatttgat gacattgtaa
     5701 ttaaaatata tgcaatatat ttttacgttt ttattaatta ggctattact attattataa
     5761 ttatcattat ttttattatt attattatta ttaaatagtc ttgcgaaata agagtgaagg
     5821 cagttgcaat gttcattgtt cccggagagt cccgcagcct ctggcctcaa cacaaagcgc
     5881 tactgtttac attaatattc atagtgctgc catggtctct gtagaggagg aacgggtggc
     5941 tcttttgttc gctagtgtca atacgtttat tcagcaaaaa gcagctcgga tttgtgaatg
     6001 cccaacaagc acacgcgcac tccaatcaaa ggacgagagg ataacgattt taaacgattc
     6061 gcaaacgtgt ttatttgtaa agacgacatc aagagcttaa tgtccacttg aaaagaaata
     6121 aatatgcccg cggcgccaag gatattttga atgtagtcgt tcatggtgag ccactcccga
     6181 attcagcact ctggagagcg accaaagagg aatagcggaa tagcaactac ttgagccatt
     6241 tctctccgtt tgatggcgtt catgattaaa aatataatca cgtgattttt tatacatata
     6301 taaatatata cataaagtaa cgctttcctt ttgagaatac ataaatattt attcgcgtga
     6361 taaatcaatg atcttatatt tatttgcacg accgaaatta gccaaattca ccaattaaaa
     6421 aaaaaataag ccaacaaaaa agaagggttt atccgttcag ttttgacttg tgtcctgttg
     6481 ttttacagct gccactgtga tcccttaagc tgcagtgaga gtgtggctaa tgccttttgt
     6541 ttaagataat cctgtaatct gttaccgaaa cggcctattg acaagccggc attcacattt
     6601 cagtcaggag gcccgtccag acatgtacat ttatgaatta cgaatgataa aattaagatc
     6661 tgcattaggc gtcagaaatg tcacgaacac ccattcattt cccacagcca ataatggatc
     6721 atgctgggaa gcgctatcct cgcagtctca aaattaatgt tgacatgttg cgtagagcta
     6781 cagattagat cagccatatg ctttatgtgt ttacctcagc ggatcttcag caagatgtgt
     6841 ttattttaag aaaatggctt cctcgctgcc tgcacagggg cattaaggaa gtgacgtgag
     6901 cgtcctcaca gtcaaattac tttatctcat gctgctttca ggcctctgct tattttatta
     6961 ttattatttt aaattagggg gaatcacggt ggcgttgtgg gtagggtagc gcgatcacct
     7021 tacagcaaga aggtcgctgg ttcgagctct ggctgggtca gttggcattt ctgtgtggag
     7081 tttaaattga attgaaattg aataaactaa attggcccta gtgtatgtgt gtgaatgtaa
     7141 gtgtgcatgg gtgtttccca gtgttgggtt tgactggaag agcatccgct tcgtaaaaca
     7201 tatgctggat aagttggcgg ttcatttcac tgtggtgacc gctgattaat aaagggacta
     7261 agctgaaaat gaaatgaatt aatacatttt aaatgagctt ttgctgacat gtttatgatt
     7321 acaaatagtg aaattgtatg ttaatttacg ataagtgcat tgttctaaat tcatttttat
     7381 gtagcagctg gtttttcttc catttgtgtc attcaacaga acatttttct aaaaaagtga
     7441 acttaaattt tctcctccct ctgacaaggc ttgatggtaa tttcagccga ctctaattac
     7501 agactcacta aaaggaatcg gtgcagtcat ttatctttat cagcgcgctg gtgggaaggt
     7561 aataggtttt gcttatgagt tgtttgcgtg cgagtctggg tccttgtgta ggcgatgctg
     7621 atgcactgca ttaaatattg agggagaagt cgctcaaaaa tagcaaagta caggctgggg
     7681 caggaacgtc aaagcccgct cagtggccac gtttcttgtt tcgtaagtcc tatttttaat
     7741 agcgataatt caggctcagt agaaggaact ccagcactat aagcagtctc tcgcctgtct
     7801 cggcgagtta tgtattttgg gatgaagtgc tcttccacct ctacctctgc tgtgctggcg
     7861 ccagtatctg ggctgtaaga tagtgtacgt gtcagtctgt gtggccgaag agcctgtgtg
     7921 ttgtgtaact cactacattc gtcgctgtgg atggctgact tgccatgggg aggtttactt
     7981 cagttcacga atcacattcg ccacgatctt gtcactttgt gtcaagactc gctctctgtg
     8041 tgtcaacagc tctgctgttt gaggttgaaa ttctgaactt gagatatatc ccaggatata
     8101 ttagggctac tgccaacgga aaaagcaaat ggtgccatgg ccatttgatt gatcaaacaa
     8161 acaccctttt gcttgggtga cagtgaaatg tcagatccat gtttgtactg ctttaataac
     8221 ttgcctctct gagctttctc cttgtcagtg tcaatttgcc tgagatataa attccggttg
     8281 ccccgaaagc ttattttatt aaaaaaaagc taacatgcac tcatacaacg tgcgcacata
     8341 catgtgaggc ttgctaaaag tcaagaagca agccaatagc aagagtcgtc agactgctgt
     8401 tacctgggca acctggttag aggtaaggct caaggcaaga ataaatcatt ccactgaaga
     8461 gacacagcgt tacagcgaga tgagagcaag aggcgatagt gaagggagaa caaaagaaca
     8521 gcctacccac actagtgcag atctacctgt ttgagctact gattgttcag tttgattaga
     8581 tatcagacaa ttctgctatc tccaattgca ttgctgcatg aattcctaga aatcatttta
     8641 atcaccaaaa actagtaggg acacttgtgg cgtatatgat tctgcctgtt gattgtgggg
     8701 atccaacaga aagatcatga atgcattgtt aattaagtcg gtgagacacc ctgttccacc
     8761 ctaggggccg ttctggggaa agagtgcttt cagtcagcta aagatgacta atatgtgaac
     8821 atatattact acacatgcca atttgtacat tctggatagt taccagccct gggaaaacat
     8881 cgaaaaaaat aaatcaacat agataaaatt ggcaaatccc ttctggtacg atgcagagtg
     8941 cttgctctga gaacttgtgc attgtgacag aaagcgaccg ttaaaaactg aaagtagcat
     9001 ctacaacctg catacatctg ttctttgaaa atcccctgta ggccaaatta agcatcacgc
     9061 tgtgctccac agcaacacgg atgaaccgaa aacccagaca caaacgcaca cacttttcac
     9121 ataaacaagg tttagttatg ctagctatgc tttttcgtcc tccctttgtc atcagcggcg
     9181 agtgacgtaa cacagtttga ccctggacag cgtaaagggg gggttgggaa tgagtgaaag
     9241 gactgtgaca tctgcagggc caggggctga tggagagagc tgaagtgagg gaggtcagag
     9301 gaggaggaag ggtgggatat tgtcctcctc aggccctatc cctctcagac gcattgattt
     9361 ttaacctctc agcgagaatg ccaagcatta cacccctcaa attatggttc ctcacccaac
     9421 ctgttatatt ttccacctgc agataattac cggtatgcca gagccagcga agtctgctcc
     9481 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa
     9541 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
     9601 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
     9661 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
     9721 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
     9781 gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta aggatccacc
     9841 agtcgccacc atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg
     9901 acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga
     9961 ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag
    10021 ctcactcgag aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt
    10081 ccacatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa
    10141 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc
    10201 caaactttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
    10261 cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat
    10321 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
    10381 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg
    10441 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
    10501 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
    10561 gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt
    10621 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
    10681 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
    10741 gctggagtac aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagaggc
    10801 tttctcccta tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt
    10861 gatgcggtct ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt
    10921 agatgccgac ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa
    10981 aatgaaatac agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga
    11041 tggcaatggc tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa
    11101 gttaacagat gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca
    11161 ggtaaactac gaagagtttg tacaaatgat gacagcgaag tgaccggtgg atcctggccg
    11221 tcgttttact gtaccatcga tgatgatcca gacatgataa gatacattga tgagtttgga
    11281 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
    11341 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
    11401 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
    11461 aaatgtggta tggctgatta tgatcctcta gatcagatct gcgaagatac ggccacgggt
    11521 gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat gaatcacata
    11581 cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc aggtcaaggt
    11641 gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca atgaacatgt
    11701 ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata acatttaaat
    11761 taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa tagaatgaag
    11821 tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag taaaaagtac
    11881 ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt aattgtactc
    11941 aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta aaattactca
    12001 agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca gatccactag
    12061 ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
    12121 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    12181 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    12241 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    12301 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    12361 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    12421 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    12481 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    12541 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    12601 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    12661 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    12721 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    12781 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    12841 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    12901 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    12961 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
    13021 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    13081 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    13141 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    13201 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    13261 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    13321 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    13381 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    13441 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    13501 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    13561 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    13621 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    13681 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    13741 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    13801 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    13861 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    13921 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    13981 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    14041 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    14101 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    14161 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    14221 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    14281 gaaaagtg
//
