LOCUS       sequence-92760-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN R102W mutant .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN R102W
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..31
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15..31
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(51..74)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(93..122)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(663..682)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(664..682)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(708..912)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(737..757)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acccgggatc tgttcaggaa acagctatga ccgcggccgg ccgtttaaac cttatcgtcg
       61 tcatccttgt aatccaggat atcatttgct gccagatcct cttctgagat gagtttctgc
      121 tcgagcggcc gcgtacgcgt gtcacactgc tggaagtaga agtctgtgat gggggcattc
      181 cagccaccat tctcgggaag ctgggtgagt ctgacaggct gatcggcttc aggcaccgtg
      241 cacaggaacc agcccgggta ggcagccgac tcgaagctgg aggtgagccc catgtcccgc
      301 cagtagaagg tgaagctctt ggattccttg gcaccaagat agagctccat gatgttcact
      361 ggctctagtg ttagagtcgg ctcctgcccc accccacatg acaggcactg gcttccaccc
      421 tggacaccca ggatgacggg ggacaggctg gcatccagcc accgattggg gaccacgctg
      481 atctcttcac ctttaatgac cttccctgca tgcagccctc cagctagaag ctggttatta
      541 tgcagataaa gcaccttcaa tgccgagtcc ttcattcgga agcacagcgc cccactcagg
      601 accatggcga tcgcggcggc agatctcctc ggtaccggat ccagtcgacg aattcccggc
      661 cgccctatag tgagtcgtat tacaaaattc tgacggttca ctaaacgagc tctgcttata
      721 tagacctccc accgtacacg cctaccgccc atttgcgtca acggggcggg gttattacga
      781 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      841 tggagacttg gaaaatcccc gtgagtcaaa ccgctatcca cgcccattgg tgtactgcca
      901 aaaccgcatc accatggtaa tagcgatgac taatac
//
