LOCUS       sequence-92782-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An empty gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K
  TITLE     Genome-wide recessive genetic screening in mammalian cells with a 
            lentiviral CRISPR-guide RNA library.
  JOURNAL   Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 
            2013 Dec 23.
  PUBMED    24535568
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2800"; 
            journalName: "Nat Biotechnol"; date: "2014-03"; volume: "32"; issue:
            "3"; pages: "267-73"
COMMENT     Sequence Label: pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
       61 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
      121 agagctccca ggctcagatc tggtctaacc agagagactc tggtctaacc agagagaccc
      181 agtacaggca aaaagcagat cttgacttct ttgggagtga attagccctt ccagtccccc
      241 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg
      301 tacctagcgg ccgctcaatt aagcttgtgc cccagtttgc tagggaggtc gcagtatctg
      361 gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt tggcctcctt gattctttcc
      421 agtctgtagt ccacatagta gacgccaggc atcttgaggt tcttagcggg tttcttggat
      481 ctatatgtgg tcttgatgtt tgcgatcaga tggctcccgc ccacgagctt cagggccatg
      541 tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg tctcggtgaa ggcctcccag
      601 ccgagtgttt tcttctgcat cacagggccg ttggatgtga agttcacccc tctgatcttg
      661 acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt cagcacgccc
      721 ccgtcctcgt atgtggtgac tctctcccat gtgaagccct cagggaagga ctgcttgaag
      781 aagtcgggga tgccctgggt gtggttgatg aaggtcttgc tgccgtagag gaagctagta
      841 gccaggatgt cgaaggcgaa
//
