LOCUS       sequence-92787-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses avr-15 sgRNA in C.elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello
            CC
  TITLE     A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2014 May 30. pii: genetics.114.166389.
  PUBMED    24879462
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 May 30. pii: genetics.114.166389."
COMMENT     Sequence Label: pCCM937
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        127..626
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     misc_RNA        647..722
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcaa
       61 gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgcccttt
      121 gaattcctcc aagaactcgt acaaaaatgc tctgaagtag gtctcgagat caatactggg
      181 aagacgaaag tcttgcgaaa ccgattcgct gaccccagta aagtctactt cggtagccct
      241 tcccccacca cccagctcga cgacgtcgac gagtacatct acctcggtcg tcaaatcaac
      301 gcccaaaaca acttgatgcc ggaaatccac cgaagacgtc gagcagcctg ggctgcattc
      361 aatggaatca agaatgccac cgactccatc accgacaaga agattcgtgc gaatctgttc
      421 gactcaattg tccttccagc gctcacctac gtttcagaag cctggacatt caccaaagct
      481 ctatccgaac gagtacgaat cacacatgcc tcccccacaa attattgatc tacatcccca
      541 tttggtatag tgtcttacag gactctcaat atgtcagctg catgataaat ggtccctata
      601 taattgaatt gcaaatctaa atgtttgttt gcaatataag tcacccgttt tagagctaga
      661 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      721 gctttttttc aataatattc tagatatccc tttttgaaac taaaatgaaa tactctaaat
      781 ttttgagttc aatgtttcaa ctaatcttgg ttaaaggtaa attcccggag ggacgatttt
      841 aaagttgcca gagttgatta aattggtcca aaaa
//
