LOCUS       sequence-92791-e        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of preCOX4-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Vowinckel J, Hartl J, Butler R, Ralser M
  TITLE     MitoLoc: A method for the simultaneous quantification of 
            mitochondrial network morphology and membrane potential in single 
            cells.
  JOURNAL   Mitochondrion. 2015 Sep;24:77-86. doi: 10.1016/j.mito.2015.07.001. 
            Epub 2015 Jul 13.
  PUBMED    26184437
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion."; date: "2015-09"; volume: "24:77-86. doi"; pages: "
            10.1016/j.mito.2015.07.001. Epub 2015 Jul 13"
COMMENT     Sequence Label: pMitoLOC
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(44..291)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     257..275
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(301..1017)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     1019..1035
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg
       61 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
      121 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
      181 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
      241 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaccag
      301 ttatttgtac aattcatcca taccatgggt aataccagca gcagtaacaa attctaacaa
      361 gaccatgtgg tctctctttt cgtttggatc tttggataag gcagattgag tggataagta
      421 atggttgtct ggtaacaaga ctggaccatc accaattgga gtattttgtt gataatggtc
      481 agctaattga acagaaccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgatacc
      541 attcttttgt ttgtcagcca tgatgtaaac attgtgagag ttatagttgt attccaattt
      601 gtgacctaaa atgttaccat cttctttaaa atcaatacct tttaattcga ttctattaac
      661 taaggtatca ccttcaaact tgacttcagc tctggtcttg tagttaccgt catctttgaa
      721 aaaaatagtt ctttcttgaa cataaccttc tggcatggca gacttgaaaa agtcatgttg
      781 tttcatatga tctgggtatc tcgcaaaaca ttgaacacca taaccgaaag tagtgactaa
      841 ggttggccat ggaactggca atttaccagt agtacaaata aattttaagg tcaatttacc
      901 gtaagtagca tcaccttcac cttcaccgga gacagaaaat ttgtgaccat taacatcacc
      961 atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt tagacatgtc
     1021 gaggtcgacg gtatc
//
