LOCUS       sequence-92792-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of preCOX4-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Vowinckel J, Hartl J, Butler R, Ralser M
  TITLE     MitoLoc: A method for the simultaneous quantification of 
            mitochondrial network morphology and membrane potential in single 
            cells.
  JOURNAL   Mitochondrion. 2015 Sep;24:77-86. doi: 10.1016/j.mito.2015.07.001. 
            Epub 2015 Jul 13.
  PUBMED    26184437
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion."; date: "2015-09"; volume: "24:77-86. doi"; pages: "
            10.1016/j.mito.2015.07.001. Epub 2015 Jul 13"
COMMENT     Sequence Label: pMitoLOC
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(80..790)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(358..377)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     627..645
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      787..796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(806..871)
                     /codon_start=1
                     /product="mitochondrial presequence of yeast cytochrome c 
                     oxidase subunit IV (Hurt et al., 1984)"
                     /label=pre-CoxIV
                     /translation="MLSLRQSIRFFKPATRTLCSSR"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ccaccgcggt ggcggccgct tacttgtaca gctcgtccat gccgccggtg gagtggcggc
      121 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      181 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
      241 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
      301 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatagtct
      361 tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga
      421 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
      481 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
      541 tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt
      601 cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct
      661 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgtcc acggagccct
      721 ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct
      781 tgctcaccat ggtggcgact agtggtctag agctacacaa agttcttgtg gctggcttga
      841 aaaatcttat agattgacgt agtgaaagca ttgtgcttgt tatctatt
//
