LOCUS       sequence-92793-e        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of preCOX4-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Vowinckel J, Hartl J, Butler R, Ralser M
  TITLE     MitoLoc: A method for the simultaneous quantification of 
            mitochondrial network morphology and membrane potential in single 
            cells.
  JOURNAL   Mitochondrion. 2015 Sep;24:77-86. doi: 10.1016/j.mito.2015.07.001. 
            Epub 2015 Jul 13.
  PUBMED    26184437
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion."; date: "2015-09"; volume: "24:77-86. doi"; pages: "
            10.1016/j.mito.2015.07.001. Epub 2015 Jul 13"
COMMENT     Sequence Label: pMitoLOC
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    80..583
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        620..724
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(943..962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa
      121 tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat
      181 tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa
      241 ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc
      301 agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg
      361 tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa
      421 gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa
      481 aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat ttatatattt
      541 atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg
      601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      781 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      841 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      901 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      961 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1021 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1081 attatgcagt gctgccataa ccatgagtga
//
