LOCUS       sequence-92796-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of preCOX4-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Vowinckel J, Hartl J, Butler R, Ralser M
  TITLE     MitoLoc: A method for the simultaneous quantification of 
            mitochondrial network morphology and membrane potential in single 
            cells.
  JOURNAL   Mitochondrion. 2015 Sep;24:77-86. doi: 10.1016/j.mito.2015.07.001. 
            Epub 2015 Jul 13.
  PUBMED    26184437
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion."; date: "2015-09"; volume: "24:77-86. doi"; pages: "
            10.1016/j.mito.2015.07.001. Epub 2015 Jul 13"
COMMENT     Sequence Label: pMitoLOC
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..96
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(325..341)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(325..341)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(342..691)
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
ORIGIN
        1 ttgtgaccat taacatcacc atctaattca accaaaattg ggacaacacc agtgaataat
       61 tcttcacctt tagacatgtc gaggtcgacg gtatcgataa gcttgatatc gaattcggaa
      121 gagtaggcgc gcttctggaa agcctggcgg gtggtggcgt tgacgatgga ggtcatctgg
      181 gtgcgcttga gggtctggag gggggagcca gtctggatgg tgcgcttgct gacctgagca
      241 acgcggacag cagggcgggc aaccttggcg gaagcagcca tccgggaggc caggcgagag
      301 gcgaggacac gagtggaggc catggatcca ctagttctag agtatggatg ggggtaatag
      361 aattgtatct atgtatctga cgaccctgta ttacacgaag gaaatagtag atgaaaagac
      421 aagagagcaa gaaaaaggaa aaacttcctt tctaacagac gctttactta accttatata
      481 tatcttattt ttttcatcga gcgtgttcaa ttggacaagg tgccaccttt tcgacacttc
      541 ggttattatg ttacacagtt ttcatgagga tggcgccttg actaacttaa ttagtcattt
      601 gccaaccacc acagttcccc aatatcgaca gcttcacgtg ccatttgcca ttttgcgatc
      661 atgtgacctc aagagaaaaa gcaaaaaata atgagagcta agggattcga agtcgacggt
      721 atcgataagc ttgatatcct tttgttgttt ccgggtgtac aatatggact tcctcttttc
      781 tggcaaccaa acccatacat cgggattcct ataatacctt cgttggtctc cctaacatgt
      841 aggtggcgga ggggagatat acaatagaac agataccaga caagacataa tgggctaaac
      901 aagactacac caattacact gcctcattga tggtggtaca taacgaacta atactgtagc
      961 cctagacttg atagccatca tcatatcgaa gtttcactac ccttttt
//
