LOCUS       sequence-92798-e        1230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-DCatch encodes Dead streptavidin (negligible biotin binding) 
            bearing a C-terminal SpyCatcher, for generation of chimeric 
            SpyAvidin tetramers .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Dead Streptavidin-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             773..790
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     gap             1052..1061
                     /estimated_length=10
     gap             1157..1170
                     /estimated_length=14
ORIGIN
        1 tnnnataatt ttgtttaact ttaagaagga gatatacata tggctgaagc tggtatcacc
       61 ggcacctggt acgcccagct gggagacacc ttcatcgtta ccgctggtgc tgacggtgct
      121 ctgaccggta cctacgaagc cgctgttggt gatgacgatg gtgacgatga cggtgatgac
      181 gatggagctg aatctagata cgttctgacc ggtcgttacg actccgctcc ggctaccgac
      241 ggttccggaa ccgctctggg ttggaccgtt gcttggaaaa acaactaccg taacgctcac
      301 tccgctacca cctggtctgg ccagtacgtt ggtggtgctg aagctcgtat caacacccag
      361 tggttgttga cctccggcac caccgaagct aacgcgtgga aatccaccct ggttggtcac
      421 gacaccttca ccaaagttaa accgtccgct gcttcaggat caggatcagg agatagtgct
      481 acccatatta aattctcaaa acgtgatgag gacggcaaag agttagctgg tgcaactatg
      541 gagttgcgtg attcatctgg taaaactatt agtacatgga tttcagatgg acaagtgaaa
      601 gatttctacc tgtatccagg aaaatataca tttgtcgaaa ccgcagcacc agacggttat
      661 gaggtagcaa ctgctattac ctttacagtt aatgagcaag gtcaggntac tgtaaatggc
      721 aaagcaacta aaggtgacgc tcatatttta aaagcttgcg gncgcactcg agcaccacca
      781 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      841 cancgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      901 ttgctgaaag gaggaactat atccngattg cnaangggac gcgccctgta ncggcgcatt
      961 tagcgcgngg gngngtggtt acgcgcagcg ngaccgctac acttgccagc gncctancgc
     1021 cngctcnttt ngctttnctt cccttccttt tnnnnnnnnn ntcgncnggn tttncccgtc
     1081 aannnnnaan nngggnnnnc ctttnnggnn ccgantnnnn nctttncngn ncctnnancn
     1141 nnnaactnnn nnggngnnnn nnnnnnnnnn ggncntnnnn cnnannanng ntnnncntnn
     1201 nncnnngnnn ncncnnnnnt tannntggng
//
