LOCUS       sequence-92823-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the insertion of an intron into eGFP via BbsI sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Seow Y, Sibley CR, Wood MJ
  TITLE     Artificial mirtron-mediated gene knockdown: functional DMPK 
            silencing in mammalian cells.
  JOURNAL   RNA. 2012 Jul;18(7):1328-37. doi: 10.1261/rna.030601.111. Epub 2012 
            May 30.
  PUBMED    22647847
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.030601.111"; journalName: "RNA"; date: "2012-07"; 
            volume: "18"; issue: "7"; pages: "1328-37"
COMMENT     Sequence Label: pEGFP-Mirt
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(214..235)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     563..582
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt acttgtacag
      181 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      241 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
      301 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      361 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
      421 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      481 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      541 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tcaagtcttc tcgagaagac
      601 tacttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      661 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
      721 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      781 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
      841 acgt
//
