LOCUS       sequence-92824-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEGFP-Mirt with a short intron from NDUFS1 cloned in.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Seow Y, Sibley CR, Wood MJ
  TITLE     Artificial mirtron-mediated gene knockdown: functional DMPK 
            silencing in mammalian cells.
  JOURNAL   RNA. 2012 Jul;18(7):1328-37. doi: 10.1261/rna.030601.111. Epub 2012 
            May 30.
  PUBMED    22647847
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.030601.111"; journalName: "RNA"; date: "2012-07"; 
            volume: "18"; issue: "7"; pages: "1328-37"
COMMENT     Sequence Label: pEGFP-NAD
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(211..232)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     560..579
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
       61 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtt atctagatcc
      121 ggtggatccc gggcccgcgg taccgtcgac tgcagaattc gaagcttact tgtacagctc
      181 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
      241 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
      301 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
      361 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
      421 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
      481 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
      541 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttaagtagat tatgaaagag
      601 tcaatctcat gctgctaata aaattaaaat ctgagacaac caaaaagatt gacagaattc
      661 tactacatac cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      721 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgta
//
