LOCUS       sequence-92835-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His3 GST tagged WT p110 alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: pBacPAK p110a WT
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..714
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     672..694
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             721..744
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttttgtataa aaaaacctat aaataccatg ggtcatcatc atcaccatca cgggtcgacc
       61 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
      121 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      181 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      241 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      301 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      361 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      421 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      481 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      541 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      601 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      661 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      721 ctggaagttc tgttccaggg gcccctaagc ttggatccct gcaggcctcg agttcctcct
      781 gctatggcag acaaccttga catctgggca gtggactcac agattgcatc cgatggcgcc
      841 atatccgtcg atttccttct gcccaccggg atttatatcc agttggaagt accctcggga
      901 agctacca
//
