LOCUS       sequence-92837-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LGP2 with a mutation to motif IIa in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Bruns AM, Pollpeter D, Hadizadeh N, Myong S, Marko JF, Horvath CM
  TITLE     ATP hydrolysis enhances RNA recognition and antiviral signal 
            transduction by the innate immune sensor, laboratory of genetics and
            physiology 2 (LGP2).
  JOURNAL   J Biol Chem. 2013 Jan 11;288(2):938-46. doi: 
            10.1074/jbc.M112.424416. Epub 2012 Nov 26.
  PUBMED    23184951
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.424416"; journalName: "J Biol Chem"; date: 
            "2013-01-11- 11"; volume: "288"; issue: "2"; pages: "938-46"
COMMENT     Sequence Label: CMV-10-3xFLAG-LGP2-MIIa
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 accgtcagaa ttaaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
       61 cgattacaag gatgacgatg acaagcttgc ggccgcgatg gagcttcggt cctaccaatg
      121 ggaggtgatc atgcctgccc tggagggcaa gaatatcatc atctggctgc ccacgggtgc
      181 cgggaagacc cgggcggctg cttatgtggc caagcggcac ctagagactg tggatggagc
      241 caaggtggtt gtattggtca acagggtgca cctggtgacc cagcatggtg aagagttcag
      301 gcgcatgctg gatggacgct ggaccgtgac aaccctgagt ggggacatgg gaccacgtgc
      361 tggctttggc cacctggccc ggtgccatga cctgctcatc tgcacagcag agcttctgca
      421 gatggcactg accagccccg aggaggagga gcacgtggag ctcactgtct tctccctgat
      481 cgtggtggat gagtgccacc acacgcacga ggacaccgtc ttcaacgtca tcatgagcca
      541 gtacctagaa cttaaactcc agagggcaca gccgctaccc caggtgctgg gtctcacagc
      601 ctccccaggc actggcgggg cctccaaact cgatggggcc atcaaccacg tcctgcagct
      661 ctgtgccaac ttggacacgt ggtgcatcat gtcaccccag aactgctgcc cccagctgca
      721 ggagcacagc caacagcctt gcaaacagta caacctctgc cacaggcgca
//
