LOCUS       sequence-9285-ex        4617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC383
FEATURES             Location/Qualifiers
     source          1..4617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(78..125)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             154..831
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             874..1548
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(2057..2079)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2179..2198
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2276..2294)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2362..2466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2467..2535,2536..3327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2685..2704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3498..4086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3987..4006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4240..4257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4374..4395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4410..4415,4416..4433,4434..4440)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4448..4464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4453..4475
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4472..4488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4472..4488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
      121 acttcaagat ccccggtaga cgataaggat ccgatggtgg cctcctccga ggacgtcatc
      181 aaagagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
      241 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      301 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc
      361 tccaaggcgt acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc
      421 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      481 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagttccg cggcaccaac
      541 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
      601 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
      661 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcag
      721 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
      781 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc
      841 accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacgt catcaaagag
      901 ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag
      961 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     1021 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag
     1081 gcgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc
     1141 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     1201 tcctccctgc aggacggcac gctgatctac aaggtgaagt tccgcggcac caacttcccc
     1261 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     1321 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
     1381 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcagctgccc
     1441 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     1501 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtagga attcgctagc
     1561 cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca aattttaaat
     1621 actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct
     1681 ttctaaaaaa ttaattcaat ccaaatctaa acatgagcgg ttttttttct ctttccgtct
     1741 cccaattcgt attccgctcc tctcatctga acacaatgtg caagtttatt tatcttctcg
     1801 ctttcatttc attaggacgt ggggggaatt ggtggaaggg ggaaacacac aaaaggatga
     1861 tggaaatgaa ataaggacac acaatatgca acaacattca attcagaaat atggaggaag
     1921 gtttaaaaga aaacataaaa atatatagag gaggaaggaa aactagtaaa aaataagcaa
     1981 agaaattagg cgaacgatga gaattgtcct cgcttgggcc ctttcgtctc gcgcgtttcg
     2041 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     2101 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     2161 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     2221 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     2281 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2341 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2401 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2461 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2521 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2581 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2641 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2701 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2761 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2821 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2881 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2941 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3001 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3061 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3121 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3181 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3241 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3301 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3361 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3421 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3481 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3541 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3601 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     3661 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3721 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3781 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3841 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     3901 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3961 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4021 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4081 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4141 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4201 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4261 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4321 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4381 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     4441 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     4501 aagctgtaag tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagagct
     4561 taaaaatggc tgaaatcact cacaacgatg gatacgctaa caacttggaa atgaaat
//
