LOCUS       sequence-9286-ex        7290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC78
FEATURES             Location/Qualifiers
     source          1..7290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(35..82)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             199..219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(309..331)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4730..4752)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4852..4871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4949..4967)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5035..5139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5140..5208,5209..6000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5358..5377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6171..6759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6660..6679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6913..6930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7047..7068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7083..7088,7089..7106,7107..7113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7121..7137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7126..7148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7145..7161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7145..7161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcaagctagc ttcaaaagcg ctctgaagtt cctatacttt ctagagaata
       61 ggaacttcgg aataggaact tcaagatccc cgggattggc caaaggaccc aaaggtatgt
      121 ttcgaatgat actaacataa catagaacat tttcaggagg acccttggag ggtaccgagc
      181 tcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac cggtgggtga agaccagaaa
      241 cagcacctcg aactgagccg cgatattgcc cagcgtttca acgcgctgta tggcgagatc
      301 gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      361 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      421 ccttcccaac agttgcgaag gtaagtttaa acagatccat actaactaac ttgttctgac
      481 ataattttca gcttgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
      541 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
      601 tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc
      661 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
      721 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg
      781 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggtaagttt aattaagttg
      841 atactaacta acaaagatct gattaatttt caggacagtc gtttgccgtc tgaatttgac
      901 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt
      961 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc
     1021 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat
     1081 gatgatttct cccgcgctgt actggaggct gaagtccagg taagtttaaa caggatctta
     1141 ctaactaaca tgctaacact gaattttcag atgtgcggcg agttgcgtga ctacctacgg
     1201 gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc
     1261 ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg tctgaacgtc
     1321 gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg
     1381 cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg
     1441 cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac
     1501 cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat
     1561 gtaagtttaa actattcgtt actaactaac tttaaacatt taaattttca gatcctgctg
     1621 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
     1681 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac
     1741 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
     1801 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
     1861 gggaatggta agtttaaaca gttgaatact aactaacgga gatctttgaa attttcagaa
     1921 tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc
     1981 cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg
     2041 atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa
     2101 aaatggcttt cgctacctgg agagacgcgc ccgctgattc tttgcgaggt aagtttaaac
     2161 agaactctac taactaacac attagatcct aattttcagt acgctcacgc gatgggcaac
     2221 agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc
     2281 ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
     2341 tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac
     2401 ggtctggtct ttgccgaccg cacgccgcat ccagcgtaag tttaaacaat aacctactaa
     2461 ctaacgtaga taatttaaat tttcaggctg acggaagcaa aacaccagca gcagtttttc
     2521 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
     2581 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
     2641 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
     2701 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
     2761 tggtcagaag ccgggcacat cagcgcatgg cagcagtgga ggtaagttta aacaagatcc
     2821 tactaactaa ctctacattg atgaattttc agactggcgg aaaacctcag tgtgacgctc
     2881 cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag
     2941 ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt
     3001 ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat
     3061 aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cggtaagttt
     3121 aaacaaagtt gtactaacta acgaagatct tgataatttt cagaacgctg gaaggcggcg
     3181 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
     3241 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
     3301 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
     3361 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
     3421 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
     3481 tgttttgacc gctgggatct gccattgtca gacatgtagt aagtttaaac ttgatagtac
     3541 taactaacat gtttcattta aattttcagt accccgtacg tcttcccgag cgaaaacggt
     3601 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
     3661 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
     3721 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
     3781 gactcctgga gcccgtcagt atcggcggaa ttccaactga gcgccggtcg ctaccattac
     3841 caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag
     3901 ttctactaac taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt
     3961 ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct
     4021 atttttgtta ttatcaaaaa aacttcttct taatttcttt gttttttagc ttcttttaag
     4081 tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc
     4141 gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg
     4201 ttctgtgtac acttcttatg ttttttttac ttctgataaa ttttttttga aacatcatag
     4261 aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt
     4321 tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt
     4381 tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt
     4441 tgctttttgg gggtttcccc tattgtttgt caagagtttc gaggacggcg tttttcttgc
     4501 taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga
     4561 ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt
     4621 tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt cctactagtc
     4681 ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     4741 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     4801 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     4861 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     4921 gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat
     4981 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5041 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5101 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5161 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5221 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5281 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5341 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5401 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5461 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5521 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5581 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5641 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5701 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5761 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5821 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5881 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5941 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6001 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6061 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6301 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6541 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7021 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7141 cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac
     7201 taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg
     7261 atggatacgc taacaacttg gaaatgaaat
//
