LOCUS       sequence-92860-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  TITLE     Seung Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4tdTomato
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(110..133)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(308..327)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     577..595
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      725..734
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaggaggcgg tgccggagct gccgctgccg gtgctgccgg tgccatgccc caggaacagg
       61 tggtggcggc cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag
      121 gtgatgtcca gcttggtgtc cacgtagtag tagccgggca gttgcacggg cttcttggcc
      181 atgtagatgg tcttgaactc caccaggtag tggccgccgt ccttcagctt cagggcctgg
      241 tggatctcgc ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag
      301 cccatggtct tcttctgcat tacggggccg tcggggggga agttggtgcc gcgcatcttc
      361 accttgtaga tcagcgtgcc gtcctgcagg gaggagtcct gggtcacggt caccagaccg
      421 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg
      481 taatcgggga tgtcggcggg gtgcttcacg tacgccttgg agccgtacat gaactggggg
      541 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttggcggtc
      601 tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc
      661 atggagccct ccatgcgcac cttgaagcgc atgaactctt tgatgacctc ctcgcccttg
      721 ctcaccatgg tggcggcttt cccggggtct tttgagggat gttaatagtt tttttcacat
      781 ccaagaggat caaaatgtaa aggtaatcaa cttgactttg atgaaatgca gcggtttaat
      841 ggcaccataa catgtttttt gttatattgc tcccaagggt tggagttgag cccatatgtt
      901 ctctcctcca gaggtaaaca agtgataaat ttgcgggata taatctgatt attctagaag
      961 cagagaggaa tctttgtcct cttcggacct ttgtgtctga agagacatgt cagacc
//
