LOCUS       sequence-92869-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic 
            tracing and high-level gene expression in neurons.
  JOURNAL   Nat Protoc. 2010 Mar;5(3):595-606. doi: 10.1038/nprot.2009.248. Epub
            2010 Mar 4.
  PUBMED    20203674
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2009.248"; journalName: "Nat Protoc"; date: 
            "2010-03"; volume: "5"; issue: "3"; pages: "595-606"
COMMENT     Sequence Label: cSPBN-4GFP
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagatt aagcgtctga tgagtccgtg
      121 aggacgaaac ccggcgtacc gggtcacgct taacaaccag atcaaagaaa aaacagacat
      181 tgtcaattgc aaagcaaaaa tgtaacaccc ctacaatgga tgccgacaag attgtattca
      241 aagtcaataa tcaggtggtc tctttgaagc ctgagattat cgtggatcaa tatgagtaca
      301 agtaccctgc catcaaagat ttgaaaaagc cctgtataac cctaggaaag gctcccgatt
      361 taaataaagc atacaagtca gttttgtcag gcatgagcgc cgccaaactt aatcctgacg
      421 atgtatgttc ctatttggca gcggcaatgc agttttttga ggggacatgt ccggaagact
      481 ggaccagcta tggaattgtg attgcacgaa aaggagataa gatcacccca ggttctctgg
      541 tggagataaa acgtactgat gtagaaggga attgggctct gacaggaggc atggaactga
      601 caagagaccc cactgtccct gagcatgcgt ccttagtcgg tcttctcttg agtctgtata
      661 ggttgagcaa aatatccggg caaaacactg gtaactataa gacaaacatt gcagacagga
      721 tagagcagat ttttgagaca gccccttttg ttaaaatcgt ggaacaccat actctaatga
      781 caactcacaa aatgtgtgct aattggagta ctataccaaa cttcagattt ttggccggaa
      841 cctatgacat gtttttctcc cggattgagc atctatattc agcaatcaga gtgggcacag
      901 ttgtcactgc ttatgaagac tgttcaggac tggtatcatt tactgggttc ataaaacaaa
      961 tcaatctcac cgctagagag gcaatactat atttcttcca caagaacttt gaggaagaga
     1021 taagaagaat gtttgagcc
//
