LOCUS       sequence-9287-ex        7333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC79
FEATURES             Location/Qualifiers
     source          1..7333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(78..125)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             242..262
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(352..374)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4773..4795)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4895..4914
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4992..5010)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5078..5182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5183..5251,5252..6043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5401..5420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6214..6802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6703..6722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6956..6973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7090..7111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7126..7131,7132..7149,7150..7156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7164..7180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7169..7191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7188..7204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7188..7204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
      121 acttcaagat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
      181 taacatagaa cattttcagg aggacccttg gagggtaccg agctcagaaa aaatgactgc
      241 tccaaagaag aagcgtaagg taccggtggg tgaagaccag aaacagcacc tcgaactgag
      301 ccgcgatatt gcccagcgtt tcaacgcgct gtatggcgag atcgatcccg tcgttttaca
      361 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      421 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      481 aaggtaagtt taaacagatc catactaact aacttgttct gacataattt tcagcttgaa
      541 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
      601 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta
      661 cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc cgtttgttcc
      721 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
      781 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa
      841 cgggcgctgg gtcggttacg gccaggtaag tttaattaag ttgatactaa ctaacaaaga
      901 tctgattaat tttcaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg
      961 cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg agtgacggca gttatctgga
     1021 agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc
     1081 gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt tctcccgcgc
     1141 tgtactggag gctgaagtcc aggtaagttt aaacaggatc ttactaacta acatgctaac
     1201 actgaatttt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
     1261 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
     1321 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
     1381 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
     1441 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
     1501 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
     1561 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatgtaagtt taaactattc
     1621 gttactaact aactttaaac atttaaattt tcagatcctg ctgatgaagc agaacaactt
     1681 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
     1741 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
     1801 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
     1861 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg gtaagtttaa
     1921 acagttgaat actaactaac ggagatcttt gaaattttca gaatcaggcc acggcgctaa
     1981 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga
     2041 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga
     2101 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc
     2161 tggagagacg cgcccgctga ttctttgcga ggtaagttta aacagaactc tactaactaa
     2221 cacattagat cctaattttc agtacgctca cgcgatgggc aacagtcttg gcggtttcgc
     2281 taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg
     2341 ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg
     2401 tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga
     2461 ccgcacgccg catccagcgt aagtttaaac aataacctac taactaacgt agataattta
     2521 aattttcagg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
     2581 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
     2641 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
     2701 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
     2761 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca
     2821 catcagcgca tggcagcagt ggaggtaagt ttaaacaaga tcctactaac taactctaca
     2881 ttgatgaatt ttcagactgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc
     2941 catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg
     3001 gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact
     3061 gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag
     3121 tgaagcgacc cgcattgacc ctaacgcctg ggtcggtaag tttaaacaaa gttgtactaa
     3181 ctaacgaaga tcttgataat tttcagaacg ctggaaggcg gcgggccatt accaggccga
     3241 agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc
     3301 tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga
     3361 tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc
     3421 ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg
     3481 attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga
     3541 tctgccattg tcagacatgt agtaagttta aacttgatag tactaactaa catgtttcat
     3601 ttaaattttc agtaccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg
     3661 cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta
     3721 cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac
     3781 atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc
     3841 agtatcggcg gaattccaac tgagcgccgg tcgctaccat taccaacttg tctggtgtca
     3901 aaaataatag gggccgctgt catcagagta agtttaaact gagttctact aactaacgag
     3961 taatatttaa attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct
     4021 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     4081 aaaaacttct tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     4141 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     4201 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     4261 atgttttttt tacttctgat aaattttttt tgaaacatca tagaaaaaac cgcacacaaa
     4321 ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct
     4381 gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat
     4441 ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt tgggggtttc
     4501 ccctattgtt tgtcaagagt ttcgaggacg gcgtttttct tgctaaaatc acaagtattg
     4561 atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga
     4621 gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag
     4681 aatacattcc caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt
     4741 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     4801 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     4861 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     4921 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     4981 cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5041 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5161 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5221 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5281 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5341 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5401 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5461 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5521 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5581 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5641 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5701 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5761 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5821 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5881 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5941 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6001 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6061 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6121 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6181 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6241 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6301 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6361 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6421 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6481 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6541 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6601 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6661 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6721 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6781 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6841 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6901 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6961 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7021 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7081 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7141 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     7201 tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa ctattctcat
     7261 ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata cgctaacaac
     7321 ttggaaatga aat
//
